链霉菌次级代谢产物高产菌株的构建策略

Construction Strategies of High-Yielding Streptomyces Strain of Secondary Metabolites

随着基因测序技术的发展,人类获得了大量有关链霉菌次级代谢产物合成基因簇的信息,通过合理的构建策略可以激活其中的隐性基因簇或提高基因簇的表达水平,从而获得新的链霉菌次级代谢产物,或显著提高已知次级代谢产物的发酵水平。从基因表达调控、转座子突变、合成生物学方法、组学方法等四个方面,综述了提高链霉菌次级代谢产物产量的构建策略及其研究进展。

With the development of genetic sequencing techniques, a large number of information of secondary metabolite biosynthetic gene clusters in Streptomyces have been obtained. Activating the cryptic gene clusters among them or increasing the expression of gene clusters by rational constructing genetic engineering strains, thus obtain new secondary metabolites or significantly increase the fermentation level of known secondary metabolites. This paper summarized the advances in the promotion of the Streptomyces strain secondary metabolites production through the construction strategies from gene expression regulation, transposon mutation, synthetic bio-methods, omics methods these four aspects.