补肾通络方对大鼠膝骨关节炎软骨修复机制的实验研究

Experimental study of Bushen Tongluo recipe on the mechanism of cartilage repair in rats with knee osteoarthritis

目的研究补肾通络方对大鼠膝骨关节炎软骨胰岛素样生长因子-1(insulin-like growth factor-1,IGF-1)、骨形态发生蛋白-7(bone morphogenetic protein-7,BMP-7)、骨桥蛋白(osteopontin,OPN)表达的影响,旨在探讨补肾通络方软骨修复机制,为膝骨关节炎(knee osteoarthritis,KOA)临床治疗提供实验支持。方法60只大鼠被随机分为6组:正常组、模型组、对照组(美洛昔康)和补肾通络方组(大、中、小剂量)。正常组常规饲养,其余各组在复制KOA模型后分别予美洛昔康及补肾通络方(大、中、小剂量)灌胃,干预4周,每周评价各组大鼠体重。4周后取膝关节软骨实时定量PCR(real time quantitative PCR,RT-PCR)测定IGF-1、BMP-7、OPN表达。结果给药期间大、中、小剂量组体重较模型组增加,给药结束后中、大剂量组体重与正常组比较,差异无统计学意义(P>0.05);补肾通络方大剂量组双侧后肢膝关节宽度及瞬时速度在给药结束后改善明显(P<0.05),与正常组比较,差异无统计学意义(P>0.05);补肾通络方大剂量组干预后软骨IGF及BMP-7水平升高,各给药组OPN水平均下降,差异有统计学意义(P<0.05)。结论补肾通络方通过调控IGF-1、BMP-7、OPN表达对大鼠膝骨关节炎软骨进行修复,有效改善关节活动度,实验证明是行之有效的中药组方。

Objective To study the effects of Bushen Tongluo recipe on the expression of IGF-1,BMP-7 and OPN in rat knee osteoarthritis,aiming to explore the repair mechanism of Bushen Tongluo recipe and provide experimental support for the clinical treatment of knee osteoarthritis(KOA).Methods A total of 60 rats were randomly divided into 6 groups:normal group,model group,control group(meloxicam)and Bushen Tongluo recipe group(large,medium and small doses).The normal group was routinely reared.The other groups after given the KOA model,were administered to the meloxicam and Bushen Tongluo recipe groups(large,medium and small doses)for 4 weeks.The body weight of each group was evaluated weekly.After 4 weeks,the expression of IGF-1,BMP-7 and OPN was measured by RT-PCR of knee articular cartilage.Results The weight of the large,medium and small dose groups during the administration period was higher than that of the model group.There was no difference between the weight of the medium and large dose groups after the administration(P>0.05);The knee width and in-stantaneous velocity of bilateral hind limbs improved significantly in the high-dose group of Bushen Tongluo recipe at the end of administration(P<0.05).Compared with the normal group,the difference was not statistically significant(P>0.05).The high-dose group of Bushen Tongluo recipe increased the levels of IGF and BMP-7,and the OPN level decreased in each drug-administered group(P<0.05).Conclusion Bushen Tongluo recipe can repair rat knee osteoarthritis by regulating the expression of IGF-1,BMP-7 and OPN,and effectively improve joint mobility.The experiment proves that it can be an effective Chinese medicine prescription.