丙酸睾酮对精索静脉曲张大鼠睾丸和精子的修复作用

Effect of testosterone propionate supplement on testis and sperm of rats with varicocele

目的探讨丙酸睾酮补充治疗对精索静脉曲张青春期大鼠睾丸和精子的修复作用。方法将30只青春期SD雄性大鼠按照随机数字表法分为假手术组、模型组、低剂量组、中剂量组、高剂量组,通过结扎左肾静脉建立精索静脉曲张大鼠模型,假手术组、模型组大鼠每天注射16mg/kg生理盐水,低剂量组、中剂量组、高剂量组大鼠每天分别注射丙酸睾酮2、4、16mg/kg,连续注射14d。观察各组大鼠精子活力、精子存活率、精子畸形率、脏器质量指数等相关指标。结果模型组大鼠精子活力明显低于假手术组,中剂量组、高剂量组大鼠精子活力明显高于模型组,差异均有统计学意义(P<0.05);低剂量组与中剂量组精子存活率明显高于模型组,差异有统计学意义(P<0.05);中剂量组睾丸结构有一定的恢复;中剂量组、高剂量组附睾指数明显高于模型组,差异均有统计学意义(P<0.05);高剂量组肾脏指数明显大于假手术组,差异有统计学意义(P<0.05)。结论补充中剂量的丙酸睾酮可提高精索静脉曲张大鼠精子活力和存活率,降低脏器指数,并可能通过此作用而达到改善精索静脉曲张大鼠精子质量,提高生育力的目的。

Objective To study the effect of testosterone on the testis and sperm of rats with varicocele.Methods A total of 30 SD rats were randomly divided into 5 groups including sham-operated group,model group,low-dose group,middle dose-group and high-dose group.Varicocele was induced by ligaturing left renal vein stenosis,rats in the sham-operated group and the model group were injected with 16 mg/kg of normal saline every day,and rats in the low-dose group,the medium-dose group and the high-dose group were injected with 2,4 and 16 mg/kg of testosterone propionate respectively every day for 14 days.The sperm motility,survival rate,abnormality rate and viscera quality index were observed and compared in the five groups.Results The sperm motility in the model group was obviously lower than that in the sham-operation group,and which in the middle-dose group and high-dose group were significantly higher than that in the sham-operation group(P<0.05).The sperm survival rate in the middle-dose group and low-dose group were significantly higher than that of the model group(P<0.05).The structure of testis in the middle-dose group recovered in some degree.The testis index in the middle-dose group and high-dose group were significantly higher than that of model group(P<0.05).The kidney index in the high-dose group was significantly higher than that of model group(P<0.05).Conclusion Treated with the middle dose of testosterone propionate,the sperm motility and survival in rats with varicocele could be improved,and visceral index could be reduced,so this way might achieve the purpose of improving sperm quality and fertility.