五味子乙素对人乳腺癌细胞MCF-7增殖和Notch-1信号通路的影响

Effects of Schisandrin B on Cell Proliferation and Notch-1 Signaling Pathway in Breast Cancer Cell MCF-7

目的观察五味子乙素对人乳腺癌细胞增殖及Notch-1信号通路的影响,探讨其抑制乳腺癌发展的可能分子机制。方法不同浓度五味子乙素(0、20、40、80μmol/L)处理乳腺癌细胞MCF-7,作用24、48、72h后,CCK-8法检测MCF-7细胞增殖。裸鼠皮下接种MCF-7细胞建立裸鼠乳腺癌移植瘤模型,随机分为对照组和五味子乙素组,每4d测量肿瘤体积并绘制肿瘤生长曲线,32d后取肿瘤称重。Westernblot检测MCF-7细胞和肿瘤组织Notch-1及其下游分子Hes-1、Hey-1蛋白水平;免疫组化检测小鼠肿瘤组织Notch-1的表达。结果与空白组比较,五味子乙素显著抑制MCF-7细胞生长,且抑制作用具有时间和浓度依赖性,同时显著降低MCF-7细胞Notch-1及其下游分子Hes-1、Hey-1蛋白的表达。与对照组比较,五味子乙素组裸鼠肿瘤体积及质量明显受抑制,且肿瘤组织Notch-1及其下游分子Hes-1、Hey-1蛋白表达显著降低。结论五味子乙素可抑制乳腺癌发展,其机制可能与抑制乳腺癌细胞MCF-7及其裸鼠模型移植瘤的生长、降低Notch-1信号通路相关因子表达有关。

Objective To explore the effects of schisandrin B on the growth of MCF-7 and Notch-1 signaling pathway in breast cancer;To explore possible molecular mechanism that inhibit the development of breast cancer.Methods Human breast cancer cell MCF-7 was treated with different concentrations of schisandrin B(0,20,40,80μmol/L)for 24,48,72 h.CCK-8 assay was used to detect cell growth.Nude mice breast cancer xenograft models were established by subcutaneous inoculation of MCF-7 cells in nude mice and were randomly divided into the control group and the schisandrin B treatment group.Tumor volume was measured every 4 days and the tumor growth curve was drawn.After 32 days,the tumor was weighed.The expressions of Notch-1,Hes-1,Hey-1 in MCF-7 cells and tumor tissues were detected by Western blot.Immunohistochemistry was used to detect the expression of Notch-1 in tumor tissues.Results Compared with the blank group,schisandrin B significantly inhibited the growth of MCF-7 cells in a time-and concentration-dependent manner,and significantly decreased the expression of Notch-1 and its downstream molecules Hes-1 and Hey-1 in MCF-7 cells.Compared with the control group,the tumor volume and quality of the schisandrin B treatment group were significantly inhibited,and the expression of Notch-1 and its downstream molecules Hes-1 and Hey-1 significantly decreased.Conclusion Schisandrin B can inhibit the growth of breast cancer,and its mechanism can be related to inhibiting the growth of breast cancer cells MCF-7 and its nude mouse model,and decreasing the expression of Notch-1 signaling pathway.