多指标权重分析法结合正交试验优选补脾养肾颗粒的水提工艺

Optimization of Water Extraction Technology for Bupi Yangshen Granules Based on Multi-index Weighting Analysis Method Combined with Orthogonal Test

目的:优选补脾养肾颗粒的水提工艺,为其后续开发研究提供依据。方法:分别采用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法和高效液相色谱-二极管阵列检测(HPLC-DAD)法测定补脾养肾颗粒的水提液中黄芪甲苷和丹酚酸B的含量,以黄芪甲苷、丹酚酸B的含量以及出膏率的综合评分为指标,采用层次分析(AHP)法、指标重复性相关(CRITIC)法和AHP-CRITIC混合加权法确定各指标权重系数,设计L9(34)正交试验筛选补脾养肾颗粒的水提工艺中煎煮时间、加水倍数和煎煮次数,并进行验证试验。结果:以AHP-CRITIC混合加权法确定的权重系数最为合理,筛选的提取工艺为药材煎煮2次,每次加12倍量水、煎煮1 h。3次验证试验结果显示,黄芪甲苷、丹酚酸B的平均含量分别为8.79、609.50 mg(整方121 g药材提取得到的总量),平均出膏率为31.24%,平均综合评分为96.59分(RSD=1.01%,n=3)。结论:优选的水提工艺重复性好、稳定可行,可为补脾养肾颗粒的后续开发和工业化生产提供科学依据。

OBJECTIVE:To optimize the water extraction technology of Bupi yangshen granules,and to provide basis for the follow-up research and development of it. METHODS:The contents of astragaloside Ⅳ and salvianolic acid B in water extract of Bupi yangshen granules,were determined by HPLC-ELSD and HPLC-DAD. Using the comprehensive score of contents of astragaloside Ⅳ and salvianolic acid B and extract yield as index,weight coefficient of indicators were determined by AHP,CRITIC and AHP-CRITIC mixed weighting method. L9(34)orthogonal test was used to optimize decoction time,water volume and decoction times in water extraction technology of Bupi yangshen granules. Validation test was also performed. RESULTS:The weight coefficient determined by AHP-CRITIC mixed weighting method was the most reasonable. The optimal extraction technology was decocting twice,adding 12-fold water,1 h each time. The results of 3 times of validation test showed that the average contents of astragaloside Ⅳ and salvianolic acid B were 8.79,609.50 mg(total amount of 121 g medicinal herbs extracted from whole prescription),respectively. The average extract yield was 31.24%. Average comprehensive score was 96.59(RSD=1.01%,n=3). CONCLUSIONS:The optimized water extraction technology is reproducible,stable and feasible. It can provide a scientific basis for the follow-up development and industrial production of Bupi yangshen granules.