投喂频率和水平对长吻鮠幼鱼消化酶和肝胰脏脂肪代谢酶活性的影响

Effects of Feeding Frequency and Level on the Digestive Enzymes and Fat Metabolism Enzyme in the Hepatopancreas of Juvenile Leiocassis longirostris

【目的】研究不同投喂频率(2次/d、3次/d)和水平(即每日投喂量占试验鱼体重的比例,2%、4%、6%、8%、10%)对长吻鮠幼鱼消化酶和肝胰脏脂肪代谢酶活性的影响,为长吻鮠的人工养殖提供更健康的养殖模式。【方法】采用SPSS 17.0软件进行双因素方差分析后采用Duncan多重比较检验,显著水平为0.05。【结果】(1)当每日投喂频率为3次、投喂水平为6%时,长吻鮠幼鱼消化酶中的肠道蛋白酶、肠道淀粉酶和胰蛋白酶的活性最高,显著高于每日投喂频率为2次、投喂水平为10%的试验组以及每日投喂频率为3次、投喂水平为2%和10%的试验组(P<0.05)。(2)当每日喂频率为3次、投喂水平为6%时,肝胰脏脂肪代谢酶中的肉毒碱脂酰转移酶(CACT)、乙酰辅酶A羧化酶(ACC)、脂蛋白酯酶(LPL)和肝脂酶(HL)的活性最高,显著高于每日投喂频率为2次、投喂水平为2%的试验组以及每日投喂频率为3次、投喂水平为8%和10%的试验组(P<0.05)。【结论】最适宜投喂频率和水平分别是3次/d和6%,更适合长吻鮠幼鱼的健康养殖。

[Purpose]The effects of feeding frequency (2 times/day, 3 times/day) and feeding level (the ratio of daily feeding to experimental fish body weight, 2%, 4%, 6%, 8% and 10%, respectively) on digestive enzymes and hepatic pancreatic fat metabolism enzyme activities of juveniles Leiocassis longirostris were studied, which provided a healthier breeding mode for artificial breeding of L. longirostris.[Method]Two-way analysis of variance was performed using SPSS 17.0 software and Duncan multiple comparison test was used. The significant level was 0.05.[Result](1) The activities of intestinal protease, intestinal amylase and trypsin of juveniles with the 3 times/day feeding frequency and 6% feeding level were the highest, which was significantly higher than those of the treatment with feeding frequency twice a day and 10% feeding level, the treatments with feeding frequency 3 times/day combined feeding level 2% and 10%, respectively (P<0.05).(2) The activities of fat metabolism enzymes hepatopancreas of carnitine acyl transferase (CACT), acetyl coenzyme A carboxylase (the ACC), lipoprotein ester enzyme (LPL) and hepatic lipase (HL) with the 3 times/day feeding frequency and 6% feeding level were the highest, which was significantly higher than those of the treatment with feeding frequency twice a day and 2% feeding level, the treatments with feeding frequency 3 times/day combined feeding level 8% and 10%, respectively (P<0.05).[Conclusion]The most suitable feeding frequency and level are 3 times/d and 6%, respectively, which is more suitable for the healthy breeding juveniles L. longirostris.