非洲马铃果4种吲哚类生物碱的体外抗肿瘤效果

Inhibition Effects of Four Indole Alkaloids in Voacanga africana on Tumor Cells in vitro

为比较非洲马铃果Voacanga africana 中长春胺、冠狗牙花定碱、老刺木胺、伏康京碱等4 种吲哚类生物碱的体外抗肿瘤活性,采用MTT 法分析其对SKOV3、BEL7402、SMMC7721、Changliver 四株细胞株增殖的抑制作用,并通过AO/EB 双染观察细胞凋亡的形态变化。结果显示,4 种吲哚生物碱对四株细胞株的增殖抑制现象存在剂量依赖关系。50 μg·mL^-1 老刺木胺对四株细胞株的生长抑制率均达95%以上;相同浓度下,伏康京碱仅对BEL7402、Changliver 的抑制率超过78%;冠狗牙花定碱仅对Changliver 有超过50%的增殖抑制率;长春胺对四株细胞株的增殖抑制效果不明显。经AO/EB 法染色后,四株细胞株在12.5 μg·mL^-1 老刺木胺的作用下呈现细胞核皱缩、浓聚和偏移的现象,说明老刺木胺具有明显诱导细胞凋亡的作用;50 μg·mL-1 伏康京碱仅对BEL7402 和Changliver 具有一样的效果,另两种生物碱作用的细胞株并未见有明显的细胞凋亡现象。MTT法和AO/EB 双染法表现结果一致。老刺木胺和伏康京碱两种生物碱能够诱导卵巢癌细胞和人肝细胞凋亡从而发挥抗肿瘤作用,长春胺和冠狗牙花定碱作用效果相对较弱。

To investigate the anti-tumor activities of four indole alkaloids, Vincamine, Coronaridine, Voacamine and Voacangine in Voacanga africana in vitro, the proliferation inhibition effects of SKOV3, BEL7402, SMMC7721 and Changliver cells were determined by MTT assay, and the morphological changes of apoptosis were observed by AO/EB staining. The results of MTT assay showed that there was a dose dependent relationship between the proliferation inhibition of four cell lines and the four indole alkaloids. The growth inhibition rates of 50 μg·mL^-1 Voacamine on all four cell lines were all over 95%;at the same concentration, Voacangine only inhibited BEL7402 and Changliver by more than 78%;Coronaridine had a proliferation inhibition rate of only 50% for Changliver;the effect of Vincamine on the proliferation of four cell lines was not obvious. After staining by AO/EB method, the four cell lines showed nuclear shrinkage, concentration and migration under the action of 12.5 μg·mL^-1 Voacamine, indicating that Voacamine had obvious effect of inducing apoptosis;Voacangine only had the same effect on BEL7402 and Changliver, and the other two alkaloids acting cell lines showed no obvious apoptosis. The MTT and the AO/EB staining method showed consistent results. Voacamine and Voacengine could induce apoptosis of ovarian cancer cells and human hepatocytes, and anti-tumor effects of the other two alkaloids were relatively weak.