乳腺癌T淋巴细胞和肿瘤相关巨噬细胞浸润的临床病理分析

The Correlation Analysis between the Tumor Infiltration of Tlymphocytes and Tumor-associated Macrophages and the Clinicopathological Features of Breast Cancer

目的探讨乳腺癌组织中T淋巴细胞和CD68^+肿瘤相关巨噬细胞(TAM)的浸润与乳腺癌增殖指数Ki-67表达及临床病理的相关意义。方法采用免疫组织化学法检测45例初治乳腺癌术后组织标本T淋巴细胞和CD68^+TAM的浸润及增殖指数Ki-67蛋白的表达,光学显微镜下对T淋巴细胞和CD68^+TAM计数及Ki-67表达进行分析,并分析T淋巴细胞和CD68^+TAM的浸润与临床病理参数之间的关系。结果 T淋巴细胞浸润高表达组与低表达组的14%、20%、30%Ki-67增值指数比较,差异均无统计学意义(P>0.05);T淋巴细胞浸润高表达组与低表达组的50%Ki-67增殖指数比较,差异有统计学意义(P<0.05)。CD68^+TAM浸润高表达组与低表达组的14%、20%、30%、50%Ki-67增值指数比较,差异均有统计学意义(P<0.05);T淋巴细胞浸润数量与患者年龄、组织学分级和HER2表达无关(P>0.05),与肿块大小、淋巴结转移、ER和PR有关(P<0.05);CD68^+TAM浸润数量与肿块大小、组织学分级、ER和PR无关(P>0.05),与患者年龄、淋巴结转移和HER2表达有关(P<0.05)。结论乳腺癌浸润的CD68+TAM与乳腺癌的进展密切相关,可用来预测乳腺癌的生物学行为以及乳腺癌免疫治疗的潜在干预靶点。

Objective To investigate the correlative significance between the infiltration of CD3^+ T lymphocytes and CD68+tumor-associated macrophage(TAM) in breast cancer tissues and the expression of proliferation index-Ki-67 and clinicopathological features. Methods Immunohistochemical staining was used to detect the infiltration of CD3^+ T lymphocytes, CD68^+ TAM, and the expression of Ki-67 proteinin 45 cases postoperative tissue specimens of primary breast cancer. The number of CD3^+ T lymphocytes, CD68^+ TAM and Ki-67 expression were examinated under light microscope, and to analyze the correlations between the infiltrationCD^3+T lymphocytes and CD68^+ TAM and clinicopathological parameters. Results There were no significant difference on the comparison of the infiltration of CD3^+ T lymphocytes high and low density groups when the proliferation index-Ki-67 were 14%, 20% and 30%(P>0.05), but there were significant difference when the proliferation index-Ki-67 were 50%(P<0.05). There were all obvious significant difference on the comparison of the infiltration of CD68^+ TAM high and low density groups when the proliferation index-Ki-67 were 14%, 20%, 30% and 50%(P<0.05). The number of CD3^+ T lymphocyte infiltration was not related to the age of patients, histological grade and human epidermal growth factor receptor 2(HER2)(P>0.05). However, it was associated with the size of breast cancer mass, lymph node metastasis, estrogen receptor(ER) and progestin receptor(PR)(P<0.05). The number of CD68^+ TAM infiltration was not related to the size of mass, histological grade, ER and PR(P>0.05), but it was associated with the age, lymph node metastasis and HER2(P<0.05) in breast cancer patients. Conclusion CD68^+ TAM is closely related to the progression of breast cancer and can be used to predict the biological behavior of breast cancer and potential intervention targets of breast cancer immunotherapy.