酶抑制法快速检测有机磷农药残留的比较试验

Comparative test of enzyme reagent method for rapid detection of organophosphorus pesticide residues

采用6种商品化酶抑制法快速检测试剂盒对敌敌畏、喹硫磷、敌百虫、辛硫磷4种有机磷农药进行了快速测定。结果表明,6种试剂盒对4种有机磷农药的敏感性、检出限各不一样;6种试剂盒对4种有机磷农药抑制率与农药浓度的对数线性模拟回归的线性范围也不相同;取用3.0mL的提取液和取用2.5mL的提取液抑制率差别不大,仅有个别农药在个别浓度水平下有差异。酶抑制法快速检测试剂盒对各种有机磷农药的检测敏感性不同,在实际的检测工作中,建议对抑制率≥10%的样品进行复检,同时采用色谱质谱进行了定性定量分析。

Four organophosphorus pesticides,including dichlorvos,quinthion,trichlorfon and octamphos-methyl,were rapidly determined by six reagent kits of commercial enzyme inhibition.The results showed that the reproducibility of pesticide residue in the rapid detection kit by enzyme inhibition decreased with the increase of inhibition rate.The sensitivity and detection limit of each reagent box to several pesticides were different.The inhibition rate of each reagent box for the four pesticides is not the same as the linear regression range of the logarithm of the pesticide concentration.There was little difference between 3.0 mL extraction and 2.5 mL extraction during the test,and individual pesticides differed at individual concentration levels.Since the reagent box for rapid detection of pesticide residues by enzyme inhibition method has different sensitivity for the detection of various pesticides,it is recommended that the sample with a suppression rate of≥10%be reexamined in the actual test work,and that the chromatographic mass spectrometry be used for qualitative quantification.