Xpert MTB/RIF在儿童肺结核诊断中的研究进展

Research Progress of Xpert MTB/RIF in Diagnosis of Pulmonary Tuberculosis in Children

儿童肺结核因其特殊性如临床表现缺乏特异性、痰结核分枝杆菌量少等特点,早期诊断儿童肺结核相对困难。利福平耐药实时荧光定量核酸扩增检测技术(XpertMTB/RIF)以全自动半巢式实时PCR技术为基础,以rpoB基因为靶基因,用探针杂交方法检测合成rpoB基因是否存在突变,用于检测痰或肺泡灌洗液及粪便等多种标本结核分枝杆菌及耐药情况。该技术是结核诊断领域的创新,在儿童肺结核早期诊断中具有较高价值。

Children with pulmonary tuberculosis are characterized by their specificity such as lack of specificity in clinical manifestations and low amount of M.tuberculosis.Early diagnosis of tuberculosis in children is relatively difficult.The Xpert MTB/RIF detection system is based on the fully automated semi-nested real-time PCR technology.The rpoB gene is used as a target gene to detect the presence of mutations in the synthetic rpoB gene by probe hybridization.It is used to detect Mycobacterium tuberculosis and drug resistance in a variety of specimens such as sputum or alveolar lavage fluid and feces.This technology is an innovation in the field of tuberculosis diagnosis and has a high value in the early diagnosis of tuberculosis in children.