摘要
为了研究西藏山南牦牛源大肠杆菌的生物特性,试验选用西藏山南病死牦牛肠道组织的未知菌源,进行分离培养、耐药性分析和致病性研究,并运用分子生物学方法对其进行16SrRNA鉴定、同源性比对及进化树分析。结果表明,分离菌株可致死试验家兔,致死率高达83%,其同源性与标准大肠杆菌相比,达到了97%以上。结果说明该分离菌株为大肠杆菌,具有较强的致病性,较高的病死率。
In order to study the biological characteristics of E.coli from yaks in Shannan, Tibet, in this experiment,the unknown bacterial sources of intestinal tissues of dead yaks in Shannan, Tibet, were selected for isolation, culture, drug resistance analysis and pathogenicity study, and 16S rRNA identification, homology comparison and phylogenetic tree analysis were carried out by molecular biological methods.The results showed that the isolates could kill experimental rabbits with a lethal rate of 83%, and their homology was over 97% compared with standard E.coli.The results showed that the strain was E.coli with strong pathogenicity and high mortality.
作者
王汉鼎
董海龙
孔新平
钱军
WANG Handing;DONG Hailong;KONG Xinping;QIAN Jun(Animal Science College,Tibet Agriculture&Animal Husbandry,Nyingchi Tibet,860000,China)
出处
《高原农业》
2019年第5期522-527,550,共7页
Journal of Plateau Agriculture
基金
2018年西藏自治区兽医学创新创业平台建设
国家肉牛牦牛产业技术体系(CARS-37)
西藏自治区高校临床兽医学重点实验室建设项目支持