一株西藏山南牦牛源大肠杆菌的分离鉴定及致病性研究

Isolation, Identification and Pathogenicity of Escherichia Coli from Yaks in Shannan, Tibet

为了研究西藏山南牦牛源大肠杆菌的生物特性,试验选用西藏山南病死牦牛肠道组织的未知菌源,进行分离培养、耐药性分析和致病性研究,并运用分子生物学方法对其进行16SrRNA鉴定、同源性比对及进化树分析。结果表明,分离菌株可致死试验家兔,致死率高达83%,其同源性与标准大肠杆菌相比,达到了97%以上。结果说明该分离菌株为大肠杆菌,具有较强的致病性,较高的病死率。

In order to study the biological characteristics of E.coli from yaks in Shannan, Tibet, in this experiment,the unknown bacterial sources of intestinal tissues of dead yaks in Shannan, Tibet, were selected for isolation, culture, drug resistance analysis and pathogenicity study, and 16S rRNA identification, homology comparison and phylogenetic tree analysis were carried out by molecular biological methods.The results showed that the isolates could kill experimental rabbits with a lethal rate of 83%, and their homology was over 97% compared with standard E.coli.The results showed that the strain was E.coli with strong pathogenicity and high mortality.