用于肿瘤细胞三维培养的多孔玉米蛋白微载体制备

Preparation of Porous Zein Microcarriers for Three-dimensional Culture of Tumor Cells

皮下荷瘤模型是常用的体内肿瘤模型,其构建需要高浓度的肿瘤细胞。使用了微载体的三维培养技术因能够提供更大的表面积,比传统的二维培养更容易富集大量细胞。本研究用多种方法制备了玉米蛋白多孔微载体,并选择孔结构符合要求的一种微载体用于肿瘤细胞的扩增富集,以提高皮下荷瘤模型的成瘤率。实验结果表明,细胞在微载体上有良好的贴壁能力,与用相同材料制备的无孔微载体相比,具有更高的细胞密度。该微载体有望应用于3D肿瘤模型的构建。

Transplanted tumor model is a commonly used in vivo tumor model. However, it requires a high concentration of tumor cells. Considerable cells are easier to be collected by using three-dimensional (3D) culture technology than traditional two-dimensional culture technique. In this study, zein porous microcarriers were prepared for expanding tumor cells to construct a reliable tumor model. The experimental results show that the cells have good adhesion ability on the microcarriers, and the porous microcarriers have higher cell density than the nonporous microcarriers prepared from the same material. The microcarriers can apply in the construction of transplanted tumor models.