甲状腺乳头状癌组织高尔基体磷蛋白3表达的临床病理意义

Clinicopathological significance of Golgi phosphoprotein 3 expression in papillary thyroid carcinoma

目的探讨甲状腺乳头状癌(PTC)组织中高尔基体磷蛋白3(GOLPH3)的表达及其与PTC临床病理特征和美国甲状腺协会(ATA)复发风险分级的相关性.方法采用反转录荧光定量PCR(RT-qPCR)检测福建省立医院2017年3月至2018年4月期间手术的30例PTC患者癌组织及相应癌旁组织GOLPH3 mRNA的表达水平.采用免疫组化方法,检测福建省立医院2013年1月至2018年4月期间收治的135例PTC患者癌组织及相应癌旁组织中GOLPH3蛋白的表达,并分析其与临床病理特征及ATA复发风险分级的关系.结果PTC组织GOLPH3的mRNA表达水平高于癌旁组织(7.53±1.32比3.64±1.44,P<0.001).免疫组化结果显示,GOLPH3蛋白在PTC组织中的表达水平高于癌旁组织[66(30,95)比34(20,72),P<0.001].单因素分析结果显示,GOLPH3蛋白表达水平与PTC肿瘤大小(P=0.026)、腺外侵犯(P=0.016)、淋巴结转移(P=0.001)、TNM分期(P=0.027)相关.多因素logistic回归分析结果显示,肿瘤大小(OR=3.58,95%CI:1.19~15.46,P=0.017)和淋巴结转移(OR=7.28,95%CI:2.43~10.08,P=0.002)与GOLPH3表达水平具有独立相关性.GOLPH3表达水平与ATA复发风险分级呈正相关(P=0.041).结论GOLPH3过表达与PTC不良预后相关,检测GOLPH3表达水平有助于判断PTC增殖转移潜能及评估PTC患者的术后复发风险.

Objective To investigate the expression of Golgi phosphoprotein 3(GOLPH3)in papillary thyroid carcinoma(PTC)and its relationship with clinicopathological characteristics of PTC and American Thyroid Association(ATA)risk of recurrence stratification.Methods The mRNA expression level of GOLPH3 in PTC tissues and the matched adjacent noncancerous tissues from 30 cases of PTC undergoing surgical operation in Fujian Provincial Hospital between March 2017 and April 2018 was detected by reverse transcription-quantitative PCR(RT-qPCR).The protein expression of GOLPH3 in PTC tissues and the matched adjacent noncancerous tissues of 135 cases of PTC between January 2013 and April 2018 was measured by immunohistochemistry.The correlation between the expression of GOLPH3 in PTC and clinicopathologic characteristics and ATA risk of recurrence stratification was analyzed.Results The mRNA level of GOLPH3 in PTC tissues was significantly higher than that in adjacent noncancerous tissues(7.53±1.32 vs 3.64±1.44,P<0.001).The protein expression level of GOLPH3 in PTC tissues was significantly higher than that in adjacent noncancerous tissues[66(30,95)vs 34(20,72),P<0.001].The expression of GOLPH3 was significantly correlated to the tumor size(P=0.026),extrathyroid invasion(P=0.016),lymph node metastasis(P=0.001)and TNM stage(P=0.027)in PTC.Multivariate logistic regression analysis showed that GOLPH3 expression was independently correlated to tumor size(OR=3.58,95%CI:1.19-15.46,P=0.017)and lymph node metastasis(OR=7.28,95%CI:2.43-10.08,P=0.002).The expression of GOLPH3 was positively correlated to ATA risk of recurrence stratification(P=0.041).Conclusions Overexpression of GOLPH3 is associated with the development of PTC and poor prognosis in patients with PTC.Detection of GOLPH3 expression can help evaluate proliferative and metastatic potential of PTC,as well as the risk of postoperative recurrence in patients with PTC.