ELISA法在诊断梅毒螺旋体感染中的临床检验价值

Clinical Value of ELISA in the Diagnosis of Treponema Pallidum Infection

目的探讨ELISA法在诊断梅毒螺旋体感染中的临床检验价值。方法选取该院2017年1月—2018年12月收治的梅毒螺旋体感染患者96例,分别对所有患者进行酶联免疫吸附法(ELISA)检查、甲基胺红不加热血清试验(TRUST)检查和梅毒螺旋体明胶颗粒检查(TPPA),将不同检查方法患者的结果与TPPA结果进行对比,比较两种检查方式的假阳性率、假阴性率、灵敏度和特异性。结果观察组(ELISA法)的假阳性率和假阴性率均为0.00%,对照组(TRUST)的假阳性率为28.12%、假阴性率为20.00%,对比差异有统计学意义(P<0.05);观察组方法的灵敏度和特异性均为100.00%,均显著高于对照组的90.91%和56.45%,对比差异有统计学意义(P<0.05)。可以看出ELISA法的检测结果与TPPA检测结果相同,准确性高。结论使用ELISA法对梅毒螺旋体感染患者进行检查判断,有较高的准确性,可以作为临床常规方法推广应用。

Objective To investigate the clinical value of ELISA in the diagnosis of Treponema pallidum infection. Methods A total of 96 patients with Treponema pallidum infection who were admitted to our hospital from January 2017 to December 2018 were enrolled. All patients were examined by enzyme-linked immunosorbent assay (ELISA), methylamine red-free serum test (TRUST) and Treponema pallidum gelatin particle examination (TPPA), comparing the results of patients with different examination methods with TPPA results, comparing the false positive rate, false nega-tive rate, sensitivity and specificity of the two methods. Results The false positive rate and false negative rate of obser-vation group (ELISA method were both 0.00%, the false positive rate of control group was 28.12%, and the false nega-tive rate was 20.00%. The difference was statistically significant (P<0.05);observation group of the sensitivity and specificity of the method were both 100.00%, which were significantly higher than 90.91% and 56.45% of the control group. There was statistically significant difference between the two groups (P<0.05). It can be seen that the detection result of the ELISA method is the same as the TPPA detection result, and the accuracy is high. Conclusion The ELISA method is used to examine and judge patients with Treponema pallidum infection, which has high accuracy and can be popularized and applied as a clinical routine method.