不同方法检测黏液型铜绿假单胞菌药物敏感性结果的比较

Comparison of Drug Sensitivity Results of Different Methods for Detecting Mucoid Pseudomonas Aeruginosa

目的对不同方法检测黏液型铜绿假单胞菌药物敏感性的结果进行对比研究。方法选取该院2017年1月—2018年12月收治的68例黏液型铜绿假单胞菌感染患者,共检出68株黏液型铜绿假单胞菌;分别采用药物敏感性纸片琼脂扩散法(K-B法)、仪器法、假单胞菌与非发酵菌药物敏感性试剂盒(ATB PSE5)、微量肉汤稀释法等不同方法对分离菌株的药物敏感性进行检测分析,并将微量肉汤稀释法检测结果作为标准,对各种方法的检测结果进行对比分析。结果与微量肉汤稀释法检测结果对比,K-B法检测符合率为94.1%,ATB PSE5检测符合率为89.7%,仪器法检测符合率为86.8%;各种检测方法对比,K-B法检测准确性最为突出,差异有统计学意义(P<0.05)。结论 K-B法进行黏液型铜绿假单胞菌药物敏感性检测可靠性最为显著,值得推广应用。

Objective To compare the results of different methods for detecting the drug sensitivity of mucoid Pseu-domonas. Methods 68 patients with mucoid Pseudomonas aeruginosa who were admitted to our hospital from January 2017 to December 2018 were enrolled. A total of 68 mucoid Pseudomonas aeruginosa were detected. Drug-sensitive paper agar diffusion method was used (K-B method), instrument method, Pseudomonas and non-fermentative drug sen-sitivity kit (ATB PSE5), micro-broth dilution method and other methods to detect and analyze the drug sensitivity of the isolated strain, and the micro-broth. Results The dilution test was used as standards to compare and analyze the results of various methods. The results were compared with the results of micro-broth dilution method. The coincidence rate of KB method was 94.1%, the coincidence rate of ATB PSE5 was 89.7%, and the coincidence rate of instrumental detec-tion was 86.8%. The accuracy of KB method was the most prominent, and the difference was statistically significant(P< 0.05). Conclusion The K -B method is the most reliable for the detection of mucoid Pseudomonas aeruginosa drug sensitivity, which is worthy of popularization and application.