山西省2015-2016年食源性疾病主动监测的病原学特征分析

Active etiological surveillance for foodborne diseases in Shanxi Province,2015-2016

目的了解山西省食源性疾病主动监测中沙门菌、志贺菌、副溶血性弧菌、致泻性大肠埃希菌(包括粘附性大肠埃希菌EAEC、致病性大肠埃希菌EPEC、侵袭性大肠埃希菌EIEC、产毒性大肠埃希菌ETEC)的病原谱、耐药情况及分子分型。方法对2015-2016年山西省食源性疾病主动监测病例中分离到的沙门菌、志贺菌、副溶血性弧菌、致泻性大肠埃希菌进行血清学分型、药物敏感性试验以及PFGE分型。结果 2015-2016年共采集腹泻病人标本2 505例,检出目标菌数157例,检出率为6.27%,其中致泻性大肠埃希菌82例(3.27%)、沙门菌51例(2.04%)、志贺菌14例(0.56%)、副溶血性弧菌10例(0.40%)。致泻性大肠埃希菌中,分离率最高的是EPEC。在对15种常见抗生素药敏实验中,致泻性大肠埃希菌对亚胺培南全部敏感(100%),对头孢他啶和甲氧苄啶\磺胺甲恶唑敏感率大于90%。沙门菌以肠炎沙门菌和鼠伤寒沙门菌2个血清型为主,沙门菌对亚胺培南全部敏感(100%),对头孢西丁和头孢他啶敏感率大于90%。副溶血性弧菌毒力基因全部为tdh阳性。结论山西省2015-2016年食源性疾病主动监测中,检出率最高的是致泻性大肠埃希菌,其次为沙门菌,志贺菌,副溶血性弧菌。

To analyze the pathogenic types,drug resistance and molecular characteristics of Escherichia coli (including Enteroaggregative E.coli EAEC,Pathogenic E.coli EPEC,Enteroinvasive E.coli EIEC,Enterotoxigenic E.coli ETEC),Salmonella,Shigella and Vibrio parahaemolyticus isolated from surveillance once of food borne disease cases in Shanxi Province.The strains of Salmonella,Shigella,Vibrio parahaemolyticus and 4 kinds of E.coli isolated from food-borne diseases surveillance during 2015-2016 were collected to conduct serotyping,drug resistance test and pulsed-field gel electrophoresis (PFGE).A total of 157 stains of pathogens were isolated from 2505 diarrhea patients during 2015-2016,the overall positive rate was 6.27%.including 82 strains of E.coli (3.27%),51 strains of Salmonella spp(2.04%),14 strains of Shigella (0.56%)and 10 strains of Vibrio parahaemolyticus(0.40%).The most prevalent E.coli strains were EPEC.In the study of 15 common antibiotic drugs,all E.coli strains were sensitive to IPM (100.0%),The sensitivity rates of CFX and TMP/SMZ were greater than 90%.The dominant serotypes were Salmoneslla typhimurium and Salmonella enteritids.All Salmonella strains were sensitive to IPM (100.0%),The sensitivity rates of CFX and CAZ were greater than 90%.All 10 parahaemolyticus isolates were tdh positive.In the active etiological surveillance for foodborne disesses in Shanxi during 2015-2016,the detection rate of E.coli was highest,then Salmonella and Shigella,the detection rate of Vibrio parahaemolyticas was lowest.