自身抗体及肝功能检测在自身免疫性肝病与病毒性肝炎鉴别诊断中的价值

Value of autoantibody and liver function test in differential diagnosis between autoimmune liver disease and viral hepatitis

目的观察自身抗体及肝功能检测在自身免疫性肝病与病毒性肝炎鉴别诊断中的价值。方法选择2016-2018年我院接收的自身免疫性肝病患者60例作为观察组,病毒性肝炎患者60例作为对照组。两组患者应用间接免疫荧光法、免疫印迹法分别进行自身抗体测定、肝功能检测。比较分析两组的检测结果。结果观察组ANA、AMA-M2、SLA/LP、gp210、sp100阳性率高于对照组(P<0.05)。观察组AST、ALT、ALP、GGT、IgG水平均高于对照组(P<0.05)。两组ANA荧光核型中均质型、颗粒型占比比较,差异显著(P<0.05);观察组ANA荧光核型以均质型、核仁型为主,而对照组以颗粒型为主。结论利用自身抗体及肝功能检测能对自身免疫性肝病、病毒性肝炎进行良好的鉴别诊断,值得临床应用推广。

Objective To observe the value of autoantibody and liver function test in differential diagnosis between autoimmune liver disease and viral hepatitis. Methods Sixty patients with autoimmune liver disease were selected as observation group, and 60 patients with viral hepatitis were selected as control group in our hospital from 2016 to 2018. Indirect immunofluorescence and Western blotting were used to detect autoantibodies and liver function in the two groups respectively. The detection results of the two groups were compared and analyzed. Results The positive rates of ANA, AMA-M2, SLA/LP, gp210 and sp100 in the observation group were higher than those in the control group (P<0.05). The levels of AST, ALT, ALP, GGT and IgG in the observation group were higher than those in the control group (P<0.05). There were signoficant differences in the proportions of homogeneous type and granular type of ANA fluorescent karyotype between the two group (P <0.05). The ANA fluorescent karyotypes in the observation group were mainly homogeneous and nucleolar type, while that in the control group was mainly granular type. Conclusion Autoantibody and liver function test can be used to differentiate autoimmune liver disease and viral hepatitis, which is worthy of clinical application and promotion.