ENPP2对血管内皮细胞增殖和迁移的影响及机制

Effects of ENPP2 on proliferation and migration of vascular endothelial cells

目的探讨焦磷酸酶/磷酸二酯酶2(ENPP2)对血管内皮细胞(HUVEC)增殖和迁移的影响及机制。方法将HUVEC细胞随机分为Ad.ENPP2组、Ad.Null组,分别感染过表达病毒Ad.ENPP2和对照病毒Ad.Null,采用qRT-PCR法检测HUVEC细胞LPA受体(LPAR1~LPAR6)、ENPP2表达,CCK-8法检测HUVEC细胞的增殖能力,Transwell法检测HUVEC细胞的迁移能力,LPA试剂盒检测HUVEC细胞LPA水平,Western blotting法检测ENPP2表达及AKT信号通路的磷酸化水平;之后将HUVEC细胞随机分为LPAR1拮抗剂Ki16198组和对照组,CCK-8法和Transwell法分别检测Ki16198干预前后HUVEC细胞增殖和迁移能力,Western blotting法检测Ki16198干预前后HUVEC细胞AKT信号通路的磷酸化水平。结果Ad.ENPP2组ENPP2 mRNA、蛋白相对表达量分别为0.186±0.004、1.261±0.080,Ad.Null组分别为0.002±0.000、0.532±0.031,两组比较P<0.05;Ad.ENPP2组HUVEC细胞增殖倍数、迁移能力及HUVEC细胞上清中LPA水平均高于Ad.Null组(P均<0.05);HUVEC细胞中LPAR1~LPAR6的相对表达量分别为0.377±0.011、0.004±0.002、0.002±0.001、0.000±0.000、0.000±0.000、0.007±0.001,其中LPAR1表达最高;与Ad.Null组相比,Ad.ENPP2组AKT表达升高(P<0.05);Ki16198干预后HUVEC细胞的增殖倍数与对照组相比差异无统计学意义(P>0.05),但HUVEC细胞的迁移能力明显降低(P<0.05);与对照组相比,Ki16198干预后HUVEC细胞AKT表达下降(P<0.05)。结论ENPP2可促进HUVEC细胞的增殖与迁移,其机制可能通过激活AKT信号通路实现。

Objective To detect the effect and mechanism of ectonucleotide pyrophosphatase/phosphodiesterase 2(ENPP2)on proliferation and migration in vascular endothelial cells(HUVEC).Methods HUVEC were randomly divided into the Ad.ENPP2 group and Ad.Null group,respectively,which were infected with overexpressing virus Ad.ENPP2 and control virus Ad.Null.The expression of ENPP2 was evaluated by qRT-PCR and Western blotting.Cell proliferation was assessed by CCK-8 assay and cell migration was performed by transwell chambers after adenovirus-mediated ENPP2 gene transduction.Concentration of lysophosphatidic acid(LPA)in supernatant of HUVEC cells transduced with Ad.ENPP2 or control vector was determined by ELISA.The expression of LPA receptors(LPAR1~LPAR6)was tested by qRT-PCR.The expression of P-AKT/AKT was determined by Western blotting.After that,HUVEC were randomly divided into the ki16198 group and control group,and the cell proliferation and cell migration were assessed by CCK-8 assay and transwell chambers.Results The ENPP2 mRNA and protein was 0.186±0.004 and 1.261±0.080,respectively,in the Ad.ENPP2 group versus 0.002±0.000 and 0.532±0.031,respectively,in the Ad.Null group(both P<0.05).The migration,proliferation,and LPA level of Ad.ENPP2 group were significantly higher than those of Ad.Null group(all P<0.05).The expression of LPAR1-LPAR6 in the HUVEC was 0.377±0.011,0.004±0.002,0.002±0.001,0.000±0.000,0.000±0.000,and 0.007±0.001,with the highest receptor of LPAR1;compared with Ad.Null group,AKT expression increased in the Ad.ENPP2 group(P<0.05);no significant difference was found in the proliferation ratio of HUVEC after Ki16198 intervention as compared with that of the control group(P>0.05),but the migration ability of HUVEC decreased significantly(P<0.05).Compared with the control group,AKT expression decreased after Ki16198 intervention in the HUVEC(P<0.05).Conclusion ENPP2/LPA could enhance the migration of HUVEC through activating AKT signal pathway.