补肺汤对慢传输型便秘模型大鼠结肠内5-HT和SP的影响

Effects of Bufei Decoction on 5-ht and SP in Colon of Rats with Slow Transit Constipation

目的:观察补肺汤对慢传输型便秘(STC)模型大鼠结肠内五羟色胺(5-HT)、P物质(SP)表达的影响,为基于“肺与大肠相表里”理论从肺论治STC提供动物实验依据。方法:Sprague-Dawley(SD)大鼠30只随机分成空白对照组(A组)、模型对照组(B组)、补肺汤治疗组(C组)各10只,B、C组采用复方地芬诺酯片(DC)灌胃建立STC模型,建模后C组用补肺汤灌胃治疗,后用活性炭推进实验,检测各种大鼠肠道传输功能。用免疫组化检测各组大鼠结肠组织内5-HT、SP的表达情况。结果:补肺汤治疗组大鼠首粒黑便排出时间为(318.2±25.1)min,24h大便数量为(12.7±2.6)粒,24h大便湿重(34.9±4.4)g,各项分别与模型对照组比较差异无统计学意义(P>0.05),与空白对照组比较差异有统计学意义(P<0.05);补肺汤治疗组大鼠结肠组织内5-HT平均光密度为(0.159±0.011),SP平均光密度为(0.156±0.006),均高于模型对照组(P<0.05)。结论:补肺汤可调控STC模型大鼠结肠内5-HT、SP的表达水平。

Objective To observe the effect of Bufei Decoction on the expression of 5-hydroxy tryptamine(5-HT)and substance P(SP)in colon of STC model rats,and to provide animal experimental basis for treating STC from the lung based on the theory of"the lung and large intestine".Methods SD rats were randomly divided into blank control group(group A),model control group(group B)and Bufei Decoction treatment group(group C),with 10 rats in each group.The STC rat model was established by DC gavage in groups B and C.The rats in group C were treated with Bufei Decoction.After that,activated carbon propulsion experiment was used to detect various intestinal transmission functions of rats.Immunohistochemistry was used to detect the expression of 5-HT and SP in colon tissues of rats in each group.Results In group C,the first black defecation was excreted for(318.2±25.1)min,the number of feces at 24 h was 12.7±2.6,and the wet weight of feces at 24 h was(34.9±4.4)g.The mean optical density of 5-HT(0.159±0.011)and SP(0.156±0.006)in the colon tissues of rats treated with Bufei Decoction were both higher than that of the model control group(P<0.05).Conclusion Bufei Decoction can regulate the expression levels of 5-HT and SP in the colon of STC model rats.