HBV cccDNA检测技术与临床应用

Clinical application of covalently closed circular DNA detection techniques

共价闭合环状DNA(cccDNA)是HBV复制的原始模板,是HBV持续性感染的基础,只有彻底清除HBVcccDNA,才能真正清除HBV,因此HBVcccDNA的检测对评价慢性乙型肝炎抗病毒疗效、病毒清除、明确隐匿性HBV感染、研究乙型肝炎致病机制以及新药的研发等方面具有重要的意义,而建立灵敏度高且特异度好的HBVcccDNA检测技术是实现其临床应用价值的基础。基于文献报道,结合团队相关研究工作,对HBVcccDNA检测方法的发展及其临床应用进行综合评述。

Covalently closed circular DNA (cccDNA) is the original template for hepatitis B virus (HBV) replication and is the basis of persistent HBV infection.Only when HBV cccDNA is completely eliminated can HBV be eradicated.Therefore,the detection of HBV cccDNA plays an important role in evaluating the antiviral outcome of chronic hepatitis B,assessing HBV clearance,clarifying occult HBV infection,studying the pathogenesis of hepatitis B,and developing new drugs,and HBV cccDNA detection techniques with high sensitivity and specificity are the basis for clinical application.With reference to related reports in literature and our own research work,this article reviews the development and clinical application of HBV cccDNA detection methods in recent years.