山东省济宁市白纹伊蚊对溴氰菊酯抗性与kdr等位基因突变观察

Resistance of Aedes albopictus to deltamethrin and kdr allele mutation in Jining of Shandong Province

目的了解山东省济宁市白纹伊蚊现场群体对溴氰菊酯的抗药性,并检测击倒抗性(kdr)等位基因突变情况,为kdr突变作为杀虫剂抗性检测的生物标记提供理论依据。方法WHO成蚊接触筒法测定白纹伊蚊对溴氰菊酯的抗性,PCR扩增电压门控钠离子通道(VGSC)基因部分片段,测序后检测kdr等位基因突变情况。结果WHO成蚊接触筒检测济宁白纹伊蚊雌蚊死亡率为44.58%,在VGSC基因1534氨基酸位点检测到3种等位基因类型:野生型TTC(F1534)(71.43%)和突变型TCC(F1534S)(13.69%)、TTG(F1534L)(14.88%);检测到5种等位基因型:野生型纯合子TTC/TTC,突变型纯合子TCC/TCC、TTG/TTG,以及野生/突变型杂合子TTC/TTG、TTC/TCC。结论济宁市白纹伊蚊对溴氰菊酯已产生抗药性,并监测到其1534氨基酸位点上存在2种kdr等位基因点突变,可能与杀虫剂的长期选择压力有关。

Objective To understand the resistance of Aedes albopictus to deltamethrin in Jining,Shandong Province, and provide theoretical basis for kdr mutation as a biomarker for insecticides resistance detection. Methods The resistance of Aedes albopictus to deltamethrin was determined by the standard WHO resistance tube bioassay. PCR was used to amplify part of VGSC gene fragment, and kdr allele gene mutation was detected after sequencing. Results The mortality of female Aedes albopictus in Jining detected by the standard WHO resistance tube bioassay was 44. 58%. Three allelic were detected at the 1 534 amino acid locus of the VGSC gene: wild-type TTC ( F1534)( 71. 43%), mutant TCC ( F1534S)( 13. 69%) and ( F1534L)( 14. 88%);five genotypes were detected: homozygous wild-type TTC/TTC,homozygous mutant TCC/TCC and TTG/TTG,as well as wild /mutant heterozygotes TTC/TTG and TTC/ TCC. Conclusion Aedes albopictus has developed resistance to deltamethrin,and two kdr allelic mutations were monitored at its 1534 amino acid locus,which may be related to the long-term selection pressure of insecticides.