杏仁中氰化物测定的方法探讨

Discussion on the Determination of Cyanide in Almond

目的:完善杏仁中氰化物测定的检测步骤,对现有方法进行优化,寻找更合适的检测方法.方法:采用GB 5009.36-2016《食品安全国家标准食品中氰化物的测定》和GB/T 5750.5-2006《生活饮用水标准检验方法无机非金属指标》中4种氰化物的检测方法.结果:用GB 5009.36-2016进行实验,发现测定杏仁试样在加入显色剂后都出现不同程度的浑浊,严重干扰实验的测定.将显色剂更换为异烟酸-巴比妥酸,可消除苯甲醛浑浊造成的干扰.结论:更改显色剂后,校正曲线线性关系良好,方法准确度和精密度达标.

Objective: Improve the detection steps of cyanide in almond, optimize the existing methods and find a better detection method. Methods: Adopt GB 5009.36-2016 and GB/T 5750.5-2006 were used to test 4 determination of cyanide. Results: In the experiment with GB 5009.36-2016, we found that the almond samples were turbid to varying degrees after adding chromogenic agent, which seriously interfered with the determination of the experiment. The interference of benzaldehyde was eliminated by replacing the chromogenic reagent with isonicotinic acid-barbituric acid. Conclusion: After changing the developer, the linearity of the calibration curve is better, the accuracy and precision of the method are up to standard.