摘要
[Objectives] This study was conducted to establish a characteristic fingerprinting research method for the chemical constituents of the Zhuang medicine Pholidota cantonensis Rolfe.and its characteristic fingerprint of chemical constituents,in order to identify P.cantonensis.[Methods] Ten batches of P.cantonensis from different habitats in Guangxi and out of Guangxi (Shaoguan of Guangdong Province) were determined.The materials were extracted in 80% methanol ultrasonically for 1.5 h under the material-to-liquid ratio of 2.5 g/80 ml.The HPLC determination was carried out using an Agilent 1260 HPLP instrument equipped with an Agilent5 TC-C18 (2) column at the column temperature of 30 ℃,the wavelength of 280 nm and the injection volume of 10 μl using acetonitrile-0.4% phosphoric acid as the mobile phase and chlorogenic acid as the reference peak,and the detection took 58 min.[Results] The characteristic fingerprint of the chemical constituents of P.cantonensis were obtained,and the three chromatographic peaks of gallic acid,chlorogenic acid and syringaldehyde were compared.[Conclusions] This study can provide reference for the identification of the Zhuang medicine P.cantonensis and provide guarantee for the clinical use of Chinese medicine.
[Objectives] This study was conducted to establish a characteristic fingerprinting research method for the chemical constituents of the Zhuang medicine Pholidota cantonensis Rolfe. and its characteristic fingerprint of chemical constituents, in order to identify P. cantonensis. [Methods] Ten batches of P. cantonensis from different habitats in Guangxi and out of Guangxi(Shaoguan of Guangdong Province) were determined. The materials were extracted in 80% methanol ultrasonically for 1.5 h under the material-to-liquid ratio of 2.5 g/80 ml. The HPLC determination was carried out using an Agilent 1260 HPLP instrument equipped with an Agilent5 TC-C18(2) column at the column temperature of 30 ℃, the wavelength of 280 nm and the injection volume of 10 μl using acetonitrile-0.4% phosphoric acid as the mobile phase and chlorogenic acid as the reference peak, and the detection took 58 min. [Results] The characteristic fingerprint of the chemical constituents of P. cantonensis were obtained, and the three chromatographic peaks of gallic acid, chlorogenic acid and syringaldehyde were compared. [Conclusions] This study can provide reference for the identification of the Zhuang medicine P. cantonensis and provide guarantee for the clinical use of Chinese medicine.
基金
Supported by Collaborative Innovation Center for Zhuang and Yao Medicines(GJKY[2013]20)
Guangxi Key Laboratory of Zhuang and Yao Medicine(GKJZ[2014]32)
Guangxi Key Discipline of Zhuang Medicine(GJKY[2013]16)
Masters Innovation Project of Postgraduate Education Innovation Program of Guangxi University of Chinese Medicine(YJSP201738)
Guangxi Key Discipline of Traditional Chinese pharmacology(0501802815)
Development of Zhuangyao Huotanmu Capsule for the Treatment of Hepatitis B(NKF[2018]11)
