摘要
[Objectives] This study was conducted to establish the quality control standards for Pandanus tectorius Soland.[Methods] Microscopic identification and thin layer chromatography were used to identify and determine the moisture,total ash and extract contents.[Results] The root transection is near round.The outmost side is velamen.There are nearly 10 columns of collenchyma tissue cells inside.In the cortex,there are a large number of fiber bundles scattered,and there are also large mucous cells scattered.The endodermis is distinct,and the cells are small and closely arranged.The pericycle is distinct and forms a ring.The pericycle fiber bundles are arranged in a radial shape by more than 40 bundles of phloem and xylem alternately.The leaf transection is V-shaped.The collenchyma lies under the epidermis.The spongy tissue has many calcium oxalate square crystal and needle crystals.The mucous cells contain calcium oxalate square crystal and needle crystals.The thin layer identification indicated that in the chromatogram of each test sample,spots of the same colors appeared at the positions corresponding to the chromatogram of the reference medicinal material,and the reproducibility was good.The moisture contents of the 10 batches of samples were all below 8.0%.The total ash was below 8.0%.The acid-insoluble ash was below 2.0%.With 50% ethanol as the solvent,the extract contents were all over 12.5%.[Conclusions] The microscopic identification results are reliable and can be used as the identification basis of P.tectorius.The thin layer identification method and the test results can provide a basis for quality control of P.tectorius.
[Objectives] This study was conducted to establish the quality control standards for Pandanus tectorius Soland. [Methods] Microscopic identification and thin layer chromatography were used to identify and determine the moisture, total ash and extract contents. [Results] The root transection is near round. The outmost side is velamen. There are nearly 10 columns of collenchyma tissue cells inside. In the cortex, there are a large number of fiber bundles scattered, and there are also large mucous cells scattered. The endodermis is distinct, and the cells are small and closely arranged. The pericycle is distinct and forms a ring. The pericycle fiber bundles are arranged in a radial shape by more than 40 bundles of phloem and xylem alternately. The leaf transection is V-shaped. The collenchyma lies under the epidermis. The spongy tissue has many calcium oxalate square crystal and needle crystals. The mucous cells contain calcium oxalate square crystal and needle crystals. The thin layer identification indicated that in the chromatogram of each test sample, spots of the same colors appeared at the positions corresponding to the chromatogram of the reference medicinal material, and the reproducibility was good. The moisture contents of the 10 batches of samples were all below 8.0%. The total ash was below 8.0%. The acid-insoluble ash was below 2.0%. With 50% ethanol as the solvent, the extract contents were all over 12.5%. [Conclusions] The microscopic identification results are reliable and can be used as the identification basis of P. tectorius. The thin layer identification method and the test results can provide a basis for quality control of P. tectorius.
基金
Supported by Study on Quality Evaluation and Standards of Guangxi Zhuang Medicine(MZY2013017)
Guangxi Key Laboratory of Zhuang Yao Medicine(GKJZ[2014]32)
Zhuang Yao Medicine Collaborative Innovation Center(GJKY[2013]20)
