淫羊藿药材的UPLC指纹图谱研究

UPLC fingerprint of Epimedium herbs

[目的]建立淫羊藿药材超高效液相色谱(UPLC)指纹图谱分析方法。[方法]采用Agilent 1290超高效液相色谱仪,以CORTECS■C18(2.1 mm×100 mm,1.6μm)色谱柱,乙腈-0.1%甲酸水进行梯度洗脱,流速为0.3 m L/min,柱温为40℃,进样量为1μL,检测波长为270 nm。[结果] 12个不同批次淫羊藿药材有21个共有峰,其中有6个已知峰,分别为木兰花碱、朝藿定A、朝藿定B、朝藿定C、淫羊藿苷和宝藿苷I,不同批次的样品与标准指纹图谱相似度均在0.911以上。[结论]本实验建立的UPLC指纹图谱分析方法稳定、精密度高,可应用于淫羊藿药材的质量控制和评价。

[Objective] Establishing UPLC fingerprint analysis method for Epimedium medicinal materials.[Methods] Gradient elution with a CORTECS■C18 column (2.1 mm×100 mm,1.6 μm) in acetonitrile-0.1% formic acid water using an Agilent 1 290 series ultra performance liquid chromatography with a flow rate of 0.3 mL/min and a column temperature of 40 ℃,the injection volume is 1 μL,and the detection wavelength is 270 nm.[Results] There are 21 common peaks in 12 batches of different kinds of epimedium medicinal herbs,including 6 known peaks,namely magnoflorine,epimedin A,epimedin B,epimedin C,icariin and baohuoside I,the similarity between different batch samples and standard fingerprints is above 0.911.[Conclusion] The UPLC fingerprint analysis method established in this experiment is stable and has high precision,and can be applied to the quality control and evaluation of epimedium.