TGF-β1与MDR1在上皮性卵巢癌中的表达及相关性分析

Expression and Correlation of TGF-β1 and MDR1 in Epithelial Ovarian Cancer

目的探讨转化生长因子β1(TGF-β1)、多药耐药基因1(MDR1)在上皮性卵巢癌中的表达及临床意义。方法选取我院2015年1月1日—2017年12月31日收治的上皮性卵巢癌100例(上皮性卵巢癌组)和卵巢良性肿瘤40例(卵巢良性肿瘤组)病理组织切片,采用免疫组织化学染色法检测两组TGF-β1和MDR1的表达情况,并探讨MDR1、TGF-β1与上皮性卵巢癌临床病理特征的关系及二者阳性表达的相关性。结果上皮性卵巢癌组织中TGF-β1阳性表达率为76.00%明显高于卵巢良性肿瘤组织中的40.00%(P<0.01);在Ⅲ~Ⅳ期上皮性卵巢癌组织TGF-β1阳性表达率为88.24%高于Ⅰ~Ⅱ期癌组织中的50.00%(P<0.01)。MDR1在上皮性卵巢癌组织中的阳性表达率为72.00%高于在卵巢良性肿瘤组织中的27.50%(P<0.01);MDR1在Ⅲ~Ⅳ期及低分化上皮性卵巢癌组织中的阳性表达率明显高于Ⅰ~Ⅱ期及高中分化上皮性卵巢癌组织,差异均有统计学意义(P<0.05或P<0.01)。TGF-β1与MDR1在上皮性卵巢癌组织中的表达呈正相关(r=0.693,P<0.001)。结论TGF-β1、MDR1在卵巢肿瘤由良性向恶性发展过程中起重要的协同作用,随卵巢肿瘤恶性程度进展TGF-β1与MDR1的表达均上调,且二者表达呈正相关。

ObjectiveTo investigate the expression and clinical significance of transforming growth factor-β1 (TGF-β1) and multidrug resistance gene-1 (MDR1) in epithelial ovarian cancer (EOC).MethodsWe collected the pathological sections of 100 patients with EOC (EOC group) and 40 patients with benigh ovarian tumors (benign ovarian tumor group) who were treated from January 1, 2015 to December 31, 2017 at the Fourth Hospital of Hebei Medical University.The expression of TGF-β1 and MDR1 was detected by immunohistochemical staining.The relationship between MDR1, TGF-β1 and the clinicopathological characteristics of EOC as well as the correlation between the positive expression of the two was also discussed.ResultsThe positive expression rate of TGF-β1 in EOC group was 76.00%, which was significantly higher than 40.00% in benign ovarian tumor group ( P <0.01).The TGF-β1 positive expression rate in EOC tissues in stages Ⅲ to Ⅳ was 88.24%, which was higher than 50.00% in tissues in stage Ⅰ to Ⅱ, there was significant difference ( P <0.01).The positive expression rate of MDR1 in EOC tissue was 72.00%, which was higher than 27.50% in benign ovarian tumor tissues.The positive expression rate of MDR1 in stage Ⅲ-Ⅳ and poorly differentiated EOC tissues was significantly higher than that of stage I to Ⅱ and highly differentiated EOC tissues ( P <0.05 or P <0.01).There was a positive correlation between TGF-β1 and MDR1 expression in EOC tissues ( r =0.693, P <0.001).ConclusionTGF-β1 and MDR1 played a synergistic role in progression from benign ovarian tumors to a malignant state.With the progression of malignant ovarian tumors, the expression of TGF-β1 and MDR1 was increased, and the expression of TGF-β1 was significantly and positively correlated with that of MDR1.