天竺葵对脓毒症小鼠肾功能保护作用观察

Observation of Protective Effect of Geranium on Renal Function in Sepsis Mice

目的探讨天竺葵在脓毒症小鼠模型中对于肾脏功能的保护作用,并阐明其潜在机制。方法盲肠结扎穿孔(CLP)手术制作小鼠脓毒症模型,通过评估脓毒症小鼠模型中肾损伤、组织炎症和氧化应激指标,判断天竺葵对于脓毒症小鼠肾功能的保护作用。结果与模型组比较,天竺葵治疗后脓毒症小鼠血尿素氮(BUN)、血肌酐、尿蛋白、乳酸脱氢酶(LDH)、中性粒细胞明胶酶相关脂质运载蛋白(NGAL)、视黄醇结合蛋白(RBP)及N-乙酰-β-D-葡萄糖苷酶(NAG)水平显著降低。天竺葵还降低了白细胞介素-6(IL-6)和肿瘤坏死因子(TNF-α)在小鼠血浆的水平,降低了CLP诱导的脓毒症引起的致死率,提高了血清脂质过氧化酶水平,并通过恢复超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶活性(CAT)水平显著增强了抗氧化防御系统能力,使肾功能在脓毒症发作期间得以保护。结论天竺葵可以有效保护脓毒症小鼠的肾功能。

Objective To explore the protective effect of geranium on renal function in a mouse model of sepsis and to elucidate its underlying mechanism. Methods The sepsis of mice model were established by cecal ligation and puncture(CLP), the protective effects of geranium on renal function of the model mice were evaluated by determination of indexs of the renal impairment, tissue inflammatory and oxidative stress. Results Compared with the model group, the levels of blood urea nitrogen(BUN), serum creatinine, urinary protein, lactate dehydrogenase(LDH), neutrophil gelatinaseassociated lipocalin(NGAL), retinol-binding protein(RBP) and N-acetyl-β-D-glucosidase(NAG) in septic mice were significantly lower than those in the model group. Treatment with geranium also reduced interleukin 6(IL-6) and tumor necrosis factor-α(TNF-α) levels in mouse plasma, reduced lethal rate caused by toxicosis, increased serum lipid peroxidase levels and significantly enhanced the ability of the antioxidant defense system by restoring superoxide dismutase. The levels of glutathione peroxidase(GSH-Px) and catalase significantly enhanced the ability of antioxidant defense system and protected renal function during sepsis. Conclusion The application of geranium in sepsis mouse model can effectively protect the renal function of septic mice and provide an effective theoretical basis for the protection of renal function during sepsis.