参金复方水煎液微生物限度检查方法研究

Decoction of Dangshen and Honeysuckle comparison study on method of microbial limit test

研究参金复方水煎液的微生物限度检查法.分别采用常规法、培养基稀释法测定参金复方水煎液对金黄色葡萄球菌等3种试验菌株的回收率.参金复方水煎液常规法方法学验证的回收率为70%以上,培养基稀释法方法学验证的回收率为90%以上,均符合要求.参金复方水煎液进行微生物限度检查可采取常规和培养基稀释法.

Microbial limit test method for the study of decoction of Dangshen and Honeysuckle. The recovery rate of 3 kinds of test strain, namely, the decoction of Dangshen and Honeysuckle on staphylococcus aureus were determined by the conventional method and the culture medium dilution method. The recovery rate verified by the normal method of Shenjin compound decoction was more than 70 %, and the recovery rate verified by the medium dilution method was more than 90 %, all of which met the requirements. Conventional and medium dilution method can be adopted for microbiological limit examination of Shenjin compound decoction.