膀胱癌组织肿瘤细胞及肿瘤相关免疫细胞中PD-L1的表达及其临床意义

Expression of PD-L1 in tumor cells and tumor-associated immune cells of bladder cancer and its clinical significance

目的检测膀胱癌组织肿瘤细胞(TC)及肿瘤相关免疫细胞(IC)中细胞程序性死亡分子配体1(PD-L1)的表达,并探讨其临床意义。方法收集2018年1~12月武汉大学人民医院行手术治疗的87例膀胱癌患者手术切除组织石蜡标本,采用免疫组织化学SP263染色方法,检测标本TC和IC中PD-L1的表达情况。结果PD-L1在膀胱癌组织IC中阳性表达率高于TC,差异有统计学意义(P<0.01),二者呈正相关关系(P<0.01)。PD-L1在膀胱癌组织TC中,阳性表达与年龄、性别和病理分级有关,差异有统计学意义(P<0.05);在IC中,阳性表达与年龄、病理分级和肌层浸润有关,差异有统计学意义(P<0.05)。结论膀胱癌组织TC和IC中PD-L1表达水平的异常上调可能与膀胱癌的发生发展密切相关。

Objective To detect expressions of programmed cell death ligand 1 (PD-L1) in tumor cells (TC) and tumor-infiltrating immune cells (IC) of bladder cancer and investigate its clinical significance. Methods The paraffin-embedded specimens of 87 patients with bladder cancer undergoing surgery in Renmin Hospital of Wuhan University from January 2018 to December were collected. The expressions of PD-L1 in TC and IC were detected by immunohistochemical SP263 staining. Results The positive rate of PD-L1 expression in bladder cancer IC was higher than that in TC, and the difference was highly statistically significant (P < 0.01), while there was a positive correlation between them (P < 0.01). The positive expressions of PD-L1 in TC of bladder cancer were related to age, gender and pathological grade, and the differences were statistically significant (P < 0.05). While the positive expressions in IC were associated with age, pathological grade, myometrial invasion, and the differences were statistically significant (P < 0.05). Conclusion The abnormal up-regulation of PD-L1 expression in TC and IC of bladder cancer tissues may be closely related to the occurrence and development of bladder cancer.