锦带花水提取物镇痛作用研究

Study on the analgesic effect of extracts from Weigela florida by water

目的探讨锦带花(Weigela florida)水提取物对小鼠的镇痛作用。方法锦带花干燥药材经浸泡、提取、过滤,得锦带花水提取物。选取清洁级昆明种小鼠150只,雌雄兼用,其中50只采用热板法,随机分为锦带花水提取物小、中、大剂量组(相当于生药0.05、0.1、0.2 g/kg)、盐酸曲马多组(0.04 g/kg)和生理盐水组(等容积),每组10只,灌胃给药。测定各组给药后15、30、45、60、75和90 min小鼠的痛阈值。另100只采用扭体法致痛动物模型分别灌胃或腹腔注射给药(分组方法同前),分别测定小鼠给药后11~20 min(T1)、>20~30 min(T2)、>30~40 min(T3)、>40~50 min(T4)的扭体反应数及扭体反应抑制率。结果与生理盐水组比较,中剂量组60、75 min时及大剂量组15、45、60 min时的痛阈值明显升高,差异有统计学意义(P<0.05);与盐酸曲马多组比较,除中剂量组45、75、90 min时,大剂量组45、90 min时差异无统计学意义(P>0.05)外,其余各时间点差异均有统计学意义(P<0.05或P<0.01)。与生理盐水组比较,灌胃给药后小、大剂量组T1~T4时段,中剂量组T3和T4时段;腹腔注射给药后小、中剂量组T3、T4时段,大剂量组T2、T3、T4时段的扭体反应数均减少,差异有统计学意义(P<0.05或P<0.01)。与盐酸曲马多组比较,灌胃给药后小剂量组T1、T2时段,中剂量组T1、T2、T3时段,大剂量组T2时段;腹腔注射给药后小剂量组T1、T3时段,中剂量组T1、T2、T3时段,大剂量组T1时段的扭体反应数均明显增加,差异有统计学意义(P<0.05或P<0.01)。盐酸曲马多组灌胃或腹腔注射给药后的痛阈值与生理盐水组比较均有显著提高(P<0.05或P<0.01),扭体反应数均显著下降(P<0.01)。结论锦带花水提取物对小鼠具有一定的镇痛作用,且大剂量作用更为明显,盐酸曲马多的镇痛作用明确且显著。

Objective To explore the analgesic effect of the extracts of Weigela florida by water in mice. Methods Water extracts of Weigela florida were prepared by soak, extraction, and filtration. Animal models of pain caused by hot plate method and writhing method. A total of 150 clean-grade Kunming mice were selected, and used in both male and female, and hot plate method was used in 50 mice, they were randomly divided into small, medium and large dose groups of Weigela florida water extracts(equivalent to crude drug 0.05, 0.1, 0.2 g/kg), Tramadol Hydrochloride group(0.04 g/kg) and control group(equal volume saline), 10 mice in each group, and administered by intragastric or intraperitoneal injection. The pain thresholds of each groups at 15, 30, 45, 60, 75 min and 90 min after administration were measured. The other 100 animal models with wriggle-induced pain were treated by intragastric or intraperitoneal injection(block method was the same as before), the times of writhing response and inhibition rate of wriggle-induced pain were measured at 11-20 min(T1),>20-30 min(T2),>30-40 min(T3), and >40-50 min(T4) after administration.Results Compared with the saline group, the pain thresholds in the medium dose group at 60 and 75 min, and the large dose group at 15, 45 and 60 min increased significantly, the differences were statistically significant(P<0.05). Compared with Tramadol Hydrochloride group, there were no statistically significant differences in the medium dose group at 45, 75 and 90 min, the large dose group at 45 and 90 min(P>0.05), while the differences at other time points were statistically significant(P<0.05 or P<0.01). Compared with the saline group, the times of writhing response after the intragastric administration in the small and the large dose group at T1-T4 periods, and the medium dose group at T3 and T4 periods;the times of writhing response after the intraperitoneal injection in the small and the medium dose group at T3 and T4 periods, and the large dose group at T2, T3 and T4 periods decreased significantly, the differences were statistically significant(P<0.05 or P<0.01). Compared with Tramadol Hydrochloride group, the times of writhing response after the intragastric administration in the small dose group at T1 and T2 periods, the medium dose group at T1, T2 and T3 periods, the large dose group at T2 period;the times of writhing response after the intraperitoneal injection in the small dose group at T1 and T3 periods, the medium dose group at T1, T2 and T3 periods, the large dose group at T1 period were markedly increased, the differences were statistically significant(P<0.05 or P<0.01). Compared with the saline group, the pain threshold of the tramadol hydrochloride group was significantly increased after intragastric or intraperitoneal injection(P<0.05 or P<0.01), and the times of writhing response was significantly decreased(P<0.01). Conclusion Weigela florida water extracts have certain analgesic effects on mice, and the effect is more obvious in the large-dose group.The analgesic effect of tramadol hydrochloride is clear and significant.