HSP110家族在各发育阶段小鼠睾丸、附睾中的表达及与激素调控的关系

Expressions of HSP110 family members in the testes and epididymis of mice at different stages of development and their regulation by hormones

目的研究HSP110家族在发育中的小鼠睾丸和附睾中的表达及其是否受激素调控。方法收集不同周龄(出生后14、21、28、35、42、49、70、90d)小鼠睾丸及附睾组织,每周龄3只,应用RT-PCR检测HSP110家族成员在不同发育阶段小鼠睾丸、附睾中的基因表达水平。建立小鼠去势实验模型,应用RT-PCR检测HSP110家族基因在假手术组、去势组及去势后补充睾酮组小鼠附睾中的表达水平。结果HSP110家族成员随着发育过程的进行其基因表达水平发生变化:HSPA4、HSPA4l、HSPH1的基因在出生后不同发育阶段的小鼠睾丸中表现出先增后降,逐渐趋于平稳的趋势;HSP110家族4个成员在出生后不同发育阶段小鼠附睾中的表达也是先增后降,逐渐平稳。小鼠去势实验结果显示HSPA4和HSPA4l随着激素水平的降低其基因表达水平降低(P<0.05),补充外源性激素后其表达恢复正常。结论HSP110家族成员的基因表达水平受发育调控的影响,HSPA4和HSPA4l基因的表达受激素调控。

Objective To study the expressions of the members of HSP110 family in the testis and epididymis of mice at different stages of development and whether they are regulated by hormones.Methods The testicular and epididymis tissues of mice at different ages (14,21,28,35,42,49,70,and 90 days after birth,3 mice at each age) were collected for RT-PCR detection of the expression levels of HSP110 family members.Forty-eight mice were randomized into 3 groups for sham operation,castration, or castration with testosterone injections every other day (starting at 7 days after castration),and at 1,3,5,and 7 days after first testosterone injection,the expressions of HSP110 family in the epididymis were detected using RT-PCR.Results The mRNA expression levels of HSP110 family members underwent obvious variations with the development of the mice: HSPA4,HSPA4l and HSPH1 expressions in the testicles of the mice first increased and then decreased,and gradually became stable;they also exhibited similar temporal patterns of changes in the epididymis.In the castrated mice,the mRNA expressions of HSPA4 and HSPA4l in the epididymis decreased significantly with the reduction of serum hormone levels (P<0.05),and became normal after the supplementation of exogenous hormone.Conclusion The expression levels of HSP110 family are affected by developmental regulation,and the expressions of HSPA4 and HSPA4l are under the regulation by hormones.