红细胞膜仿生氮化硼纳米球的制备及其作为抗肿瘤药物载体的研究

Erythrocyte Membrane Biomimetic Boron Nitride Nanospheres as Carrier for the Delivery of Antitumor Drug

作者利用红细胞(Red blood cell,RBC)膜对BNNS进行包被,构建出新型仿生纳米给药系统RBC-BNNS。采用透射电镜(Transmission electron microscopy,TEM)、粒径及电位分析等手段表征并对比了红细胞膜包被前后BNNS的基础性能。在此基础上,选用阿霉素(Doxorubicin,DOX)作为抗癌药物模型,对比了细胞膜包被前后载体对阿霉素装载量的变化。选用HeLa细胞为肿瘤细胞模型,考察了RBC-BNNS的生物安全性以及DOX@RBC-BNNS的细胞摄取和体外抗肿瘤效果。

In this work,red blood cell(RBC) membrane coating technique was used to camouflage inorganic BNNS,thus forming biomimetic RBC-membrane-coated BNNS(RBC-BNNS) drug delivery system.The functionalization of BNNS with RBC-membrane was characterized by transmission electron microscopy(TEM),particle size and zeta potential analysis.Besides,the influence of RBC-membrane modification to the drug loading capacity was also analyzed with doxorubicin(DOX) as model drug.Then,the human cervical carcinoma cell line HeLa cells was selected as model cancer cells to investigate the biocompatibility of RBC-BNNS and the cellular uptake and antitumor effect of DOX@RBC-BNNS.