HPLC法同时测定舒肝健胃丸7种成分的含量

Simultaneous Determination of Seven Constituents in ShuganJianwei Pills by HPLC

建立HPLC波长切换法同时测定舒肝健胃丸中7种成分的含量。采用Hypersil ODS2 C18色谱柱,流动相为乙腈-水(梯度洗脱),检测波长为0~15min(230nm,芍药苷)、15~45min(294nm,芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、和厚朴酚、厚朴酚)。这7种成分的浓度分别在6~60、5~50、18~180、36~360、6~60、2~20、5~50μg/ml范围内与各自峰面积积分值呈良好的线性关系;平均加样回收率(n=6)为99.3%~100.6%,RSD为0.5%~1.3%。

An HPLC wavelength switching method was established for the simultaneous determination of seven constituents in ShuganJianwei pills. An Hypersil ODS2 C18 column was used, with the mobile phase of acetonitrile-water at the detection wavelength of 230 nm at 0-15 min(for paeoniflorin) and 294 nm at 15-45 min(for narirutin, naringin, hesperidin, neohesperidin, honokiol and magnolol).The method was found to be linear for the seven components in the ranges of 6-60, 5-50, 18-180, 36-360, 6-60, 2-20, and 5-50 μg/ml, respectively. The recoveries were 99.3%-100.6%, with the RSDs of 0.5%-1.3%, respectively.