TUSC3对人肝癌细胞增殖、迁移与侵袭的影响

Effect of TUSC3 on Proliferation, Migration and Invasion of Human Hepatoma Cells

[目的]探究 TUSC3对肝癌细胞(SMMC‐7721细胞系)增殖、迁移和侵袭的影响.[方法]在SMMC‐7721细胞中转染TUSC3过表达质粒,采用Real‐time PCR和Western blot技术检测 T USC3 mRNA和蛋白表达验证质粒转染效果. MTT检测转染后24 h 、48 h 、72 h和96 h时的细胞增殖情况,划痕实验检测细胞迁移,Transwell小室检测细胞侵袭.[结果]本研究成功在SMMC‐7721细胞中过表达 TUSC3 . MTT结果显示TUSC3过表达转染组的细胞增殖能力显著低于空载体转染组( P <0 .01).划痕实验结果显示TUSC3过表达转染组的细胞迁移能力也显著下降( P <0 .05).与划痕实验结果类似,Transwell结果也表明TUSC3过表达后,肝癌细胞侵袭能力下降( P <0 .01).[结论]TUSC3抑制人肝癌细胞SMMC‐7721细胞系的增殖、迁移和侵袭,可能在肝癌发展中发挥抑癌作用.

[Objective]To investigate the effect of tumor suppressor candidate 3 (TUSC3) on proliferation, migration and invasion of hepatoma cells(SMMC-7721).[Methods]TUSC3 overexpression vector was transfected into SMMC-7721 cells, and TUSC3 mRNA and protein expression were detected by Real-time PCR and Western blot to verify the transfection efficiency of plasmid. MTT was used to detect cell proliferation at 24 h, 48 h, 72 h and 96 h after transfection. The cell migration ability was detected by wound healing and the invasion ability was detected by Trans well chamber.[Results]TUSC3 was overexpressed in SMMC- 7721 successfully. The results of MTT showed that the cell proliferation in TUSC3-overexpression group was significantly lower than that in empty vector group ( P<0.01). The results of the wound healing showed that the cell migration in the TUSC3 overexpression transfection group was also significantly decreased ( P<0.05). Similar to wound healing, the results of Trans well also showed that the invasive of hepatoma cells decreased after TUSC3 overexpression ( P<0.01).[Conclusion] TUSC3 could inhibit proliferation, migration and invasion of hepatoma cells cells, and may play a tumor suppressing role in the development of hepatic carcinoma.