CTHRC1与TGF-1、Smad7在OSF组织中的表达及相关性分析

Detection and Correlation Analysis of CTHRC1, TGF-βl and Smad7 Expression in Oral Submucous Fibrosis

[目的]探讨胶原三股螺旋重复蛋白1(CTHRC1)、转化生长因子‐β1(TGF‐β1)、Smad蛋白 7 (Smad7)在口腔黏膜下纤维性变(OSF)组织中的表达及其相关性.[方法]选取33例OSF患者(早期10例,中期13 例,晚期 10 例)的新鲜颊黏膜组织及 10 例来源于外科手术的健康颊黏膜组织,免疫组化检测CT HRC1 、TGF‐β1 、smad7的表达水平, Pearson 软件分析 TGF‐β1表达与 CT HRC1和 Smad7的相关性.[结果]CT HRC1在OSF早、中、晚期病变组织中的表达均显著高于正常口腔黏膜组织( P =0 .007 ,0 .002 , 0 .006);TGF‐β1在早、中期病变组织中表达升高( P =0 .007 ,0 .031),晚期表达与对照比较差异无统计学意义( P =0 .323);Smad7在OSF各期病变组织中的表达也均高于对照组( P =0 .032 ,0 .007 ,0 .005 ).在 OSF病变组织中TGF‐β1与CT HRC1的表达呈正相关性(R=0 .678 ,P =0 .0010),TGF‐β1与Smad7的表达无明显相关性(R=0 .003 ,P =0 .9908).[结论]CT HRC1在OSF病变组织中表达增加,且与TGF‐β1的表达呈正相关,可作为抗纤维化新靶点.

[Objective]To investigate the expression of CTHRC1 (collagen triple helix repeat containing 1),TGF-β1 and smad7 in OSF (Oral Submucous Fibrosis) by immunohistochemistry in order to explore the correlation between CTHRC1 and TGF-βl/smad signaling pathway in pathogenesis of OSF.[Methods]Buccal mucous tissues were obtained from 33 OSF patients (10 cases in the early stage, 13 cases in the middle stage and 10 cases in the late stage) and 10 healthy volunteers' mucous tissues. After paraffin embedding, the expression of CTHRC1, TGF-β1 and smad7 proteins in sections were detected by immunohistochemistry. The correlations between TGF-βl expression and CTHRC1, smad7 were tested by Pearson software.[ResuIts]The expression of CTHRC1 in the early, middle and later stage OSF was significantly higher than that in normal oral mucosa( P =0.007,0.002,0.006). SO did the expression of smad7 in all stages of OSF( P =0.032,0.007, 0.005). The expression of TGF-βl was increased in the early and middle stage OSF tissues ( P = 0.007, 0.031), however, there was no significant difference between the late expression and the normal control ( P = 0.323). There was a positive correlation between TGF-βl and CTHRC1 expression in OSF lesions (R = 0.678, P =0.0010), and there was no significant correlation between TGF-pl and Smad7 expression (R = 0.003, P = 0.9908).[Conclusions]The expression of CTHRC1 increases significantly in all stages of OSF, which is positively correlated with the expression of TGF-βl. CTHRC1 can be a new target for anti-fibrosis.