珍珠贝外套膜酶解产物促进皮肤创伤愈合效果研究

Effect of enzymatic hydrolysis from mantle of pearl oyster on skin wound healing

为进一步拓宽马氏珠母贝(Pinctada martensii)的高值化利用途径,文章探讨了马氏珠母贝外套膜酶解产物体外抗菌活性、促凝血作用以及对小鼠软组织开放性创伤愈合效果。结果表明,珍珠贝外套膜酶解产物具有一定的促凝血作用,但对13种测试菌无抑制作用;涂抹给药6 d后,给药组小鼠的创伤愈合率显著高于阴性对照组(P<0.05),与阳性对照组无显著差异(P>0.05);给药组的第14天愈合率达100%,疤痕缩小率显著高于阴性对照组(P<0.05)。生化指标测试结果显示,与2个对照组相比,马氏珠母贝外套膜酶解产物能够抑制炎症因子IL-6生成;与阴性对照组相比,其可极显著促进IL-10分泌;与阴性对照组相比,给药组皮肤组织中FGF-2含量无显著性差异(P>0.05),阳性对照组EGF含量显著高于其他组(P<0.05);而TGF-β和CCND1含量均显著高于阴性对照组(P<0.05)。综上,马氏珠母贝外套膜酶解产物具有一定促凝血、抑制炎症作用并促进生长因子分泌,从而加快小鼠软组织开放性创伤愈合,对浅表瘢痕增生具有一定的抑制作用。

In order to promote the high value utilization of pearl oyster(Pinctada martensii),we were determined explore the antibacterial activity and procoagulant activity of enzymatic hydrolysis from its mantle(EHM),and the effects of EHM on open wound healing of skin of mice.Results showed that EHM possessed of procoagulant activity in mice but had no antibacterial activity against 13 tested strains.Compared with the negative control group,the healing rate of wound of mice increased significantly after skin application with EHM for 6 d(P<0.05),but there was no significant difference with comparison with the positive control group(P>0.05).The healing rate was 100%on 14th day for the drug group,and the scar contraction rate was significantly higher than that of the negative control group(P<0.05).Biochemical indicators test indicated that EHM had significant inhibitation effect on inflammatory factor IL-6(P<0.05);compared with the negative control group,it could promote IL-10 secretion in wound tissues significantly(P<0.05),but there was no significant difference in FGF-2 content in skin tissue of the drug groups(P>0.05),and EGF content in the positive control group was significantly higher than that in the other groups(P<0.05).Howerer,there was no significant difference in the contents of FGF-2 and EGF between the drug groups(P<0.05).In summary,EHM has procoagulant,anti-inflammatory and promotional effects,which can accelerate the open wound healing of soft tissue in mice and has a certain inhibitory effect on superficial scar hyperplasia.