摘要
研究抗菌肽Jelleine-I对指状青霉(Penicillium digitatum)生长的影响及可能的作用机理,并采用损伤接种的方法评价Jelleine-I对柑橘果实采后绿霉病的控制效果。结果表明,Jelleine-I能明显抑制P. digitatum的生长,其最小抑菌浓度和最小杀菌浓度分别为6.25 mmol/L和12.5 mmol/L;同时,其能有效控制柑橘果实采后绿霉病的发生。经Jelleine-I处理后的P. digitatum菌丝体细胞膜通透性增加,细胞间隔膜损坏,细胞内绿色SYTOX Green荧光强度增加,细胞间蓝色Calcofluor White荧光减少。此外,经Jelleine-I处理后的P. digitatum菌丝体胞外电导率以及胞内核酸物质泄漏量均显著增加(P<0.05)。综上,Jelleine-I通过破坏P. digitatum细胞膜结构完整性,引起胞内物质泄漏,加速P. digitatum死亡,从而有效控制柑橘果实采后绿霉病的发生。
In this study, the effect of peptide Jelleine-I(antimicrobial peptide derived from royal jelly) on the growth of Penicillium digitatum and its possible mode of action were investigated. Meanwhile its effect on the control of postharvest green mold induced by stab inoculation in citrus fruit was evaluated. The results showed that Jelleine-I could significantly inhibit the growth of P. digitatum. The minimal inhibitory concentration and minimal fungicidal concentration were 6.25 and 12.5 μmol/L, respectively. Jelleine-I could significantly decrease the incidence of green mold of citrus fruit. After being treated with Jelleine-I, the membrane permeability of P. digitatum hyphae increased and the intercellular membrane was damaged. The intracellular green SYTOX Green fluorescence increased and the intercellular blue Calcofluor White fluorescence decreased. In addition, Jelleine-I significantly increased the extracellular conductivity and the intracellular leakage of nucleic acid(P<0.05). Therefore, Jelleine-I could effectively control citrus postharvest green mold by damaging the membrane structural integrity and consequently causing intracellular material leakage and accelerating the death of P. digitatum.
作者
李心丹
王文军
邓丽莉
姚世响
曾凯芳
LI Xindan;WANG Wenjun;DENG Lili;YAO Shixiang;ZENG Kaifang(College of Food Science,Southwest University,Chongqing 400715,China;Food Storage and Logistics Research Center,Southwest University,Chongqing 400715,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第19期256-262,共7页
Food Science
基金
“十三五”国家重点研发计划重点专项(2018YFD0401301)
重庆市社会事业与民生保障科技创新专项一般项目(cstc2017shms-xdny80058)