MALDI-TOF MS技术用于细菌鉴定的研究进展

Matrix-assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry for Bacterial Strain Identification

基质辅助激光解吸电离时间质谱(MALDI-TOFMS)技术已成为微生物实验室中快速有效的细菌物种鉴定新技术。常规的细菌鉴定主要基于生化测试并且需要冗长的孵育程序,相比之下,MALDI-TOFMS可以在几分钟内直接从新生菌落中鉴定出细菌种类。目前已经开发了基于MALDI-TOFMS技术用于细菌鉴定的多种方法,并且已经在多项研究中证明了该方法的有效性。该技术还适用于对生物亚种水平的分类以及某些抗生素抗性机制的表型检测,例如β-内酰胺酶的产生等。

Matrix-assisted laser desorption/ionization-time of flight mass spectrometer(MALDI-TOF MS)is one of the most popular MS instruments used in biology,due to its rapid and precise identification of genus and species.Conventional bacterial identi cation is based essentially on biochemical tests and requires lengthy incubation procedures.By contrast,MALDI-TOF MS can identify bacterial species in a few minutes,based on identifying a characteristic spectrum of each species and then matched with a large database within the instrument.Various approaches have been developed for bacterial identification and the efficacy of this method has been demonstrated in several studies.This technique should also be suitable for the classification of organisms to subspecies level and for the phenotypic detection of certain antibiotic resistance mechanisms,such asβ-lactamases production.