PTPN9表达水平与结直肠癌的临床关系研究

Clinical relationship between PTPN9 expression and colorectal cancer

目的探究PTPN9在结直肠癌中的表达情况及其与各项临床指标关系。方法回顾性分析2007年12月—2008年12月于哈医大附属肿瘤医院就诊且病理明确、临床资料完整的结直肠癌患者,共146例。随机挑选出132例患者的肿瘤组织和14例患者的非癌组织,进行PTPN9蛋白质免疫组化染色,由两位高年资的病理科医师进行双盲评分。参照染色评分,比较患者的年龄、性别、大体形态、分化程度、预后等各项临床指标。生存分析采用Kaplan-Meier曲线,应用Cox比例风险模型进行多因素分析。结果PTPN9在肿瘤组织中呈高表达水平(P=0.009),且与N分期(P=0.021)、M分期(P=0.022)、病理分期(P=0.008)、糖尿病患病(P=0.022)和生存情况(P=0.001)有关。与低表达PTPN9的结直肠癌患者相比,高表达PTPN9的结直肠癌患者总生存期下降(P<0.001)。多因素Cox回归分析显示,PTPN9的表达水平为影响结直肠癌患者预后的独立风险因素之一(HR=1.202,P<0.05)。结论PTPN9的表达强度在癌组织与非癌组织中有统计学差异,在癌组织中的表达强度更高;随着PTPN9的表达强度增高,肿瘤病理分期也随之升高;高表达的PTPN9与糖尿病发病之间存在一定相关性;高表达PTPN9的结直肠癌患者的总生存期较短。

Objective The aim of this study was to investigate the expression of PTPN9 in colorectal cancer and its relationship with various clinical indicators.Methods A retrospective study of 146 patients with colorectal cancer who had a clear pathology and complete clinical data from December 2007 to December 2008 at Harbin Medical University Cancer Hospital was performed.Tumor tissues from 132 patients and non-cancerous tissues from 14 patients were randomly selected for determination of PTPN9 protein by immunohistochemistry,and double-blind scores were performed by two senior pathologists.According to the staining score,the clinical indicators such as age,gender,gross morphology,degree of differentiation and prognosis were compared.Survival analysis was performed by Kaplan-Meier curve using Cox proportional hazard model for multivariate analysis.Results The expression of PTPN9 in tumor tissues was statistically different from non-tumor tissues( P =0.009).There was a relationship between PTPN9 expression and N stage( P =0.021),M stage( P =0.022),pathological stage( P =0.008),clinical indicators such as diabetes( P =0.022)and survival( P = 0.001). High expression of PTPN9 would result in a decrease overall survival in patients with colorectal cancer( P <0.001).Multivariate Cox regression analysis showed that the expression of PTPN9 was one of the independent risk factors affecting the prognosis of patients with colorectal cancer( HR =1.202, P <0.05).Conclusion The expression of PTPN9 is significantly different between cancer tissues and non-cancerous tissues,and the expression of PTPN9 is higher in cancer tissues.As the expression of PTPN9 was increased,the pathological stage of tumors was also increased.The high expression of PTPN9 is a correlation with the onset of diabetes and also leads to a decrease in overall survival of patients with colorectal cancer.