基于circRNA靶点的子痫前期诊断研究

Diagnosis of preeclampsia based on circRNA target

目的研究circRNA靶点对于子痫前期的诊断价值。方法收集早发型及晚发型子痫前期孕产妇外周血及胎盘组织、子痫前期雌性C57BL/6J小鼠模型胎盘组织及本团队已有研究基础的子痫前期滋养细胞株等样本,以正常孕产妇、小鼠及滋养细胞株相应样本为对照,采用circRNA芯片或高通量测序技术,结合生物信息学手段,筛选子痫前期的特异性靶标circRNA;而后对上调或下调明显的circRNA,采用QTPCR、NorthernBlotting、FISH等现代分子生物学技术,验证靶标circRNA存在的真实性,并进行功能预测。结果小鼠子痫前期抑制基因相关mRNA、miRNA和protein的表达情况均显著增高(P<0.05)。结论circRNA过表达可以作为子痫前期的一个观察指标,与子痫前期预测标志物合用效果更好。

Objective To study the diagnostic value of circRNA target in preeclampsia. Methods The peripheral blood and placental tissue of early and late onset preeclampsia pregnant women, placental tissue of female preeclampsia C57BL/6J mice model and the sample of preeclampsia trophoblast cell line based on our team, anormal pregnant and lying-in women, mice and trophoblast lines were collected as control. Aspecific target for preeclampsia, was screened by circRNA chip or high throughput sequencing combined with bioinformatics. Then the QT-PCR, Northern Blotting, FISH and other modern molecular biology techniques were used to verify the authenticity of the target circRNA and predict the function of the upregulated or down-regulated circRNA. Results The expression of mRNA,miRNA and protein related to preeclampsia in mice was significantly increased (P<0.05). Conclusion The overexpression of circRNA can be used as an index of preeclampsia, and it is more effective in combination other predictors of preeclampsia.