濒危药材明党参愈伤组织培养条件研究

Study on the Culture Conditions of Callus of Endangered Changium smyrnioides

[目的]研究培养条件对明党参愈伤组织生长及多糖积累的影响。[方法]分别采用单因素及正交试验的方法,以明党参叶片诱导产生的愈伤组织为材料,从外植体种类、激素种类及浓度、大量母液添加量、光照条件等培养条件比较愈伤组织生长情况及多糖含量。[结果]叶片外植体、大量母液1倍添加量、0.5mg/L2,4-D及暗培养等培养条件较有利于明党参愈伤组织生长及多糖的合成。[结论]该研究为培养生产明党参愈伤组织及多糖提供理论和方法依据。

[Objective]The research aimed to study the culture conditions for the callus growth and the accumulation of amylose in Changium smyrnioides.[Method]Using single factor and orthogonal test methods,the callus induced by the leaves of the ginseng was used as the material,the callus growth and polysaccharide content were compared from the explant species, hormone type and concentration, the amount of mother liquor added, and the light conditions.[Result]The culture conditions of leaf explants, large amount of mother liquor 1 times, 0.5 mg/L 2, 4-D and dark culture were more favorable for the growth of callus and the synthesis of polysaccharides.[Conclusion]This study provides theoretical and methodological basis for the cultivation of callus and polysaccharides from Changium smyrnioides.