摘要
目的明确TPT1-AS1在胃癌及远端基本正常组织中的差异表达,探讨其表达水平与胃癌临床病理特征的相关性,评价其作为胃癌生物标志物的可能性。方法采用实时荧光定量PCR(quantitative real-time PCR, qRT-PCR)方法检测TPT1-AS1在胃癌患者的肿瘤组织(73例)及远端基本正常组织(66例)标本的表达水平。结果TPT1-AS1在胃癌组织中的表达水平较远端基本正常组织低表达,其表达水平与胃癌临床病理特征如年龄、性别、肿瘤大小、肿瘤生长部位等无相关性( P >0.05)。TPT1-AS1作为胃癌诊断生物标志物时,曲线下面积、灵敏度及特异性分别为0.641、72.6%及51.5%。生存分析结果显示,高表达患者预后优于低表达患者,但差异无统计学意义( P >0.05)。结论TPT1-AS1在胃癌组织中表达降低,具有作为胃癌诊断生物标志物的可能,本研究为胃癌的早诊、早治提供新的线索。
Objective To investigate the differential expression of TPT1-AS1 in gastric cancer and distal normal tissues, and the correlation between its expression level and clinicopathological features of gastric cancer, and to evaluate its potential as a biomarker for gastric cancer. Methods Quantitative real-time PCR (qRT-PCR) was used to detect the expression of TPT1-AS1 in tumor tissues (73 cases) and distal normal tissues (66 cases) of gastric cancer patients. Results The expression of TPT1-AS1 in gastric cancer tissues was lower than that in distal normal tissues. The expression of TPT1-AS1 was not related with the clinicopathological features of gastric cancer such as age, sex, tumor size and tumor growth site. When TPT1-AS1 was used as a biomarker for diagnosis of gastric cancer, the area under the curve, sensitivity and specificity were 0.641, 72.6% and 51.5%, respectively. Survival analysis showed that patients with high expression had better prognosis than patients with low expression, although the difference was not statistically significant ( P >0.05). Conclusion The expression of TPT1-AS1 is decreased in gastric cancer and has the potential to be a biomarker for the diagnosis of gastric cancer. This study provides new clues for early diagnosis and treatment of gastric cancer.
作者
方新鑫
孙明军
宫月华
FANG Xinxin;SUN Mingjun;GONG Yuehua(Department of Gastroenterology, and Key Laboratory of Cancer Etiology and Prevention (China Medical University), Liaoning Provincial Eudcation Department, the First Affiliated Hospital of China Medical University, Shenyang 110001, China;Tumor Etiology and Screening Department of Cancer Institute and General Surgery, and Key Laboratory of Cancer Etiology and Prevention (China Medical University), Liaoning Provincial Eudcation Department, the First Affiliated Hospital of China Medical University, Shenyang 110001, China)
出处
《胃肠病学和肝病学杂志》
CAS
2019年第10期1113-1116,共4页
Chinese Journal of Gastroenterology and Hepatology
基金
国家自然科学基金项目(81670506)
辽宁省科技计划项目(201602822)