胃癌患者癌组织及血清中lncRNA EXOC7的表达及其临床意义

The expression and clinical significance of lncRNA EXOC7 in cancer tissues and serum of patients with gastric cancer

目的探讨胃癌患者癌组织及血清中lncRNA EXOC7的表达及其临床意义。方法 选取郑州大学第五附属医院2016年1月至2018年6月收治的134例胃癌患者为研究对象,收集胃癌患者的癌组织、对应癌旁组织(距癌组织边缘≥1 cm)及血清样本,另将同期在我院体检的80名体检正常者作为对照组,采集血清样本,应用qRT-PCR技术检测组织及血清中lncRNA EXOC7的表达,并分析其与患者临床病理特征的关系。结果 qRT-PCR结果显示,胃癌组织中lncRNA EXOC7的相对表达量(8.24±1.86)明显高于癌旁组织(2.37±0.81),且差异有统计学意义( t=33.494,P =0.000);胃癌患者血清中lncRNA EXOC7的相对表达量( 4.29± 0.47)明显高于对照组(0.92±0.23),且差异有统计学意义( t=74.553,P =0.000);不同性别、年龄、肿瘤直径、肿瘤位置的患者胃癌组织及血清中lncRNA EXOC7表达水平比较,差异均无显著统计学意义( P >0.05),但胃癌患者血清及癌组织中lncRNA EXOC7在不同临床分期、浸润深度、分化程度及有无脉管癌栓、淋巴结转移中的表达比较,差异有显著统计学意义( P <0.05),即TNM分期越高、分化程度越低、浸润深度越深、有淋巴结转移、有脉管癌栓的患者,其胃癌组织及血清中lncRNA EXOC7表达水平越高。结论 lncRNA EXOC7在胃癌患者血清及癌组织中均呈高表达,其与胃癌恶性程度相关,可能成为胃癌早期诊断及病情判断的潜在指标。

Objective To investigate the expression of lncRNA EXOC7 in cancer tissues and serum of patients with gastric cancer and its clinical significance.Methods 134 patients with gastric cancer admitted to the Fifth Affiliated Hospital of Zhengzhou University from Jan. 2016 to Jun. 2018 were selected as subjects. Cancer tissues, corresponding adjacent tissues (1 cm from the margin of cancer tissue) and serum samples were collected from patients with gastric cancer. In addition, 80 healthy persons who had physical examination in our hospital during the same period were taken as the control group, and serum samples were collected. The expression of lncRNA EXOC7 in cancer tissues and serum was detected by qRT-PCR, the relationship between lncRNA EXOC7 and clinicopathological features was analyzed. Results The results of qRT-PCR showed that the relative expression of lncRNA EXOC7 in cancer gastric cancer tissues (8.24±1.86) was significantly higher than that in adjacent tissues (2.37±0.81), and the difference was statistically significant ( t= 33.494 , P =0.000). The relative expression of lncRNA EXOC7 in the serum of patients with gastric cancer (4.29±0.47) was significantly higher than that in the control group (0.92±0.23), and the difference was statistically significant ( t=74.553, P =0.000). There was no significant difference in the expression of lncRNA EXOC7 in gastric cancer tissues and serum of patients with different gender, age, tumor diameter and tumor location ( P > 0.05 ). However, the expression of lncRNA EXOC7 in serum and cancer tissues of gastric cancer patients was significantly different in different clinical stages, depth of invasion, degree of differentiation, and presence or absence of vascular thrombosis and lymph node metastasis ( P <0.05). The higher of the TNM stage, the lower of the degree of differentiation, the deeper of the infiltration depth, the lymph node metastasis, and the vascular tumor thrombus, the expression of lncRNA EXOC7 in gastric cancer tissues and serum was higher. Conclusion LncRNA EXOC7 is highly expressed in serum and cancer tissues of gastric cancer patients, which is related to the degree of malignancy of gastric cancer, which may be a potential indicator for early diagnosis and disease judgment of gastric cancer.