表观健康成人血清谷胱甘肽还原酶参考区间的建立

Establishment of the reference intervals of serum glutathione reductase in apparently healthy adults

目的建立表观健康成人血清谷胱甘肽还原酶(GR)的参考区间,分析GR与其他常用临床检测指标的相关性。方法 检测430名表观健康者(男205名、女225名)血清GR活性,根据美国临床实验室标准化协会(CLSI)C28-A3文件的相关要求,确定基于性别和年龄的GR参考区间。结果 剔除5个离群值后,表观健康者血清GR活性呈非正态分布。男性GR活性为53(47~57)U/L,略高于女性[51(47~55)U/L](Z=-2.156,P<0.05)。相关分析结果显示,血清GR活性与年龄、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、γ-谷氨酰基转移酶(GGT)、碱性磷酸酶(ALP)、葡萄糖(Glu)、三酰甘油(TG),总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)呈正相关,与高密度脂蛋白胆固醇(HDL-C)呈负相关,与总胆红素(TB)无相关性。男性各年龄组间差异无统计学意义,合并为男性组;女性≥51岁年龄组GR活性显著高于18~50岁各年龄组,因此合并为18~50岁组和≥51岁组。采用百分位数法得出表观健康成人血清GR的参考区间为男性36~72U/L,女性38~65U/L(18~50岁)和45~67U/L(≥51岁)。结论 初步建立了表观健康成人血清GR的参考区间,为GR的临床应用提供依据。

Objective To establish the reference intervals of serum glutathione reductase(GR) in apparently healthy adults. Methods Serum GR activities of 430 healthy adults,including 205 males and 225 females,were determined,and the age- and sex-related reference intervals were determined according to the Clinical and Laboratory Standards Institute(CLSI) C28-A3 document. Results After eliminating 5 outliers, the GR activities showed a non-Gaussian distribution in serum of healthy adults. Serum GR activity in males was 53(47-57) U/L,which was slightly higher than that in females [51(47-55) U/L](Z=-2.156,P<0.05). Correlation analysis showed that serum GR activities had positive correlations with age,alanine aminotransferase (ALT),aspartate aminotransferase(AST),gamma glutamyltransferase(GGT),alkaline phosphatase (ALP),glucose(Glu),triglyceride(TG),total cholesterol(TC) and low-density lipoprotein cholesterol (LDL-C)and a negative correlation with high-density lipoprotein cholesterol(HDL-C). There was no correlation between serum GR activities and total bilirubin(TB). The GR activities in males showed no statistical significance among all the age groups,and the subjects were combined into male group. The GR activities in ≥51-year-old females were higher than those in 18-50-year-old females,so that the subjects were regrouped into 18-50-year-old and ≥51-year-old groups. The reference intervals of serum GR activities in healthy adults were 36-72 U/L for males, 38-65 U/L(18-50-year-old) and 45-67 U/L(≥51-year-old) for females by percentiles. Conclusions The reference intervals of serum GR in apparently healthy adults have initially established,which provides a reference for the clinical application of GR.