胡萝卜抗冻蛋白基因克隆及对甜菜遗传转化的研究

Cloning of Gene of Carrot Antifreeze Protein and Its Transformation to Sugar Beet

甜菜虽然是比较耐寒的作物,但对零下低温较敏感,早春的晚霜和秋季的早霜冻害严重影响甜菜苗期和糖分积累期的生长.所以,培育耐寒抗冻甜菜品种对甜菜生产十分有利.本研究采用PCR的方法克隆了胡萝卜抗冻蛋白基因afp,并构建了甜菜叶绿体表达载体,利用基因枪法转化甜菜叶柄,经诱导分化、抗性筛选及对抗性植株PCR检测,获得了 3株含有抗冻蛋白基因的转基因再生植株,为快速培育耐寒甜菜品种奠定基础.

Sugar beet in our country and the world at one of the main sugar crops, in North China, Northeast China, Northwest China large -scale cultivation. Compared with the cold-sensitive plants is a relatively cold - resistant sugar beet crops, but sugar beet is more sensitive to low temperature below zero. Frostbite of late frost in early spring and early frost in autumn seriously affects the growth of sugar beet at seedling stage and sugar accu? mulation stage. Therefore, it is very beneficial for the production of northern sugar beet to cultivate the variety of cold and cold beet. In this study, the method of PCR cloning using a new antifreeze protein found in carrots (afp) gene, chloroplast expression vector construction of sugar beet, sugar beet transformed using particle bom? bardment, with the initial antifreeze protein gene in transgenic plant regeneration. Induction of differentiation, ac? cess to a number of resistant buds, many times after subculture, eventually three resistant plants. Some of them resistant plants for PCR testing, results show that: there is an integration of the chloroplast genome have afp gene. For the rapid cultivation of cold 一 resistant frozen beet varieties lay the foundation.