摘要
目的研究一种快速、简便检测AFP的方法,为有助肝癌早筛、早诊提供技术支撑。方法采用上海芯超AFP全血一步法快速检测试剂盒,对江苏启东市肝癌高发区859例HBsAg阳性的高风险人群进行AFP筛查,并与郑州安图生物AFP酶联免疫法试剂盒同步比对检测。对于AFP阳性者,进一步结合B超,观察是否已经出现肝占位性病变,从而对病情进行初步诊断。结果以郑州安图AFP检测试剂(酶联免疫法)为标准,上海芯超AFP快速检测试剂的灵敏度为95.65%(22/23),特异度为99.40%(831/836),总符合率为99.30%(853/859)。两种试剂盒检测结果具有较高的符合率。同时结合B超结果发现,在检测出AFP阳性人群中有两例出现了肝占位现象,初步判断为早期肝癌。结论AFP全血上海芯超AFP全血一步法快速检测试剂能实现AFP高风险人群家庭自测,提高肝癌的早诊率。
Objective Serum AFP is the major methods for diagnosis,relapse monitor and effect evaluation for HCC.A rapid,convenient home-test kit for AFP is necessary for the hepatitis patients,which will greatly improve the early diagnosis rate of HCC.Methods AFP one-step rapid detect kit produced by Shanghai Outdo Biotech Co.,Ltd was used to screen 859 individuals with positive HBsAg who were at-risk for HCC in Qidong,Jiangsu.The result was compared with that of the ELISA kit produced by Zhenzhou Autobio Diagnostics Co.,Ltd.According to the AFP result,combined with the B-mode ultrasonography to found placeholder,primary diagnosis could be concluded.Results The results showed that the positive accordance rate(sensitivity)and the negative accordance rate(specificity)between AFP one-step rapid detect kit and the Autobio ELISA kit were 95.65%(22/23)and 99.40%(831/836),respectively.The total diagnose accordance rate reached up to 99.30%(853/859).The screening results proved a high accordance rate of the two methods.Combined with the results of Bmode ultrasonography,two patients were primary diagnosed as early HCC.Conclusion The AFP one-step rapid detect kit had a great significance in the realization of home-detect and in the improvement of the early screening and diagnosis of HCC.
作者
金杰
张小燕
赵志东
陈建国
JIN Jie;ZHANG Xiaoyan;ZHAO Zhidong;CHEN Jianguo(National Engineering Center for Biochip at Shanghai,Shanghai 201203,China;Shanghai Eighth People′s Hospital,Shanghai 200235,China)
出处
《中国热带医学》
CAS
2019年第10期931-933,971,共4页
China Tropical Medicine
基金
卫生部“十二五”肝癌重大专项(No.2013ZX10002007001)