辣椒AP2/ERF家族转录因子CaERF109的克隆和表达分析

Cloning and Expression Analysis of AP2/ERF Transcription Factor Gene CaERF109 in Pepper

植物AP2/ERF转录因子参与多个生物学进程。为了研究辣椒AP2/ERF家族成员CaERF109在逆境和激素响应过程中的作用,从‘遵辣1号’中克隆了CaERF109,并对其基因结构、理化性质等进行生物信息学分析,利用qRT-PCR检测高温、低温、生长素、赤霉素和茉莉酸甲酯处理后CaERF109的表达情况。结果显示:CaERF109的开放阅读框长693 bp,由2个外显子组成,编码230个氨基酸,分子量为2.6 kD,等电点为7.91,含一个氨基酸组成的AP2/ERF DNA结合结构域;CaERF109与同为茄科的番茄、马铃薯、烟草同源基因具有较近的亲缘关系,氨基酸序列同源性超过76%;CaERF109基因的表达受低温诱导,不受高温和植物外源激素生长素、赤霉素和茉莉酸甲酯诱导。综上所述,CaERF109基因可能参与辣椒低温胁迫应答,更对CaERF109基因深入的研究将有利于该基因功能和调控机制的揭示。

AP2/ERF transcription factors participate in multiple biological processes.To investigate the role of pepper AP2/ERF transcription factor,CaERF109 in stress and hormone response,CaERF109 was cloned from'Zunla-1',and its structure,physicochemical properties,etc.were analyzed by bioinformatics.QRT-PCR was conducted to detect the expression of CaERF109 in Zunla-1 after treatment of high temperature,low temperature auxin(IAA),gibberellic acid(GA)and methyl jasmonate(MeJA).Results showed that CaERF109 had two exons and contained an open reading frame(ORF)of 693 bp,encoding 230 amino acid residues,which contained an amino acid AP2/ERF DNA binding domain.The molecular weight of the protein encoded by this gene was 2.6 kD and the pI value was 7.91.CaERF109 and its homologs in tomato,potato and tobacco were at the same clade and showed a closer relationship.The amino acids sequence similarities of them were over 76%.The expression level of CaERF109 could be significantly induced by low temperature treatment,but can not induced by treatment with high temperature,IAA,GA or MeJA.In conclusion,CaERF109 may be involved in pepper cold stress response,further studies will help to reveal the specific function and regulation mechanism of this gene.