菊花CmbZIP18基因的克隆及表达分析

Cloning and Expression Analysis of CmbZIP18 Gene in Chrysanthemum morifolium

bZIP转录因子蛋白在植物应对非生物胁迫过程中发挥着重要功能,但菊花中该家族基因功能报道较少。为了研究bZIP转录因子在菊花发育及非生物胁迫中的功能,本研究以菊花‘神马’为试验材料,采用荧光定量PCR方法,研究了bZIP基因CmbZIP18的基因序列和表达模式。结果表明:该基因开放阅读框长879bp,编码292个氨基酸,预测分子量约为32.76 kD,等电点为6.29。CmbZIP18含有一个保守的BRLZ结构域。亚细胞定位预测CmbZIP18蛋白定位于细胞核。利用保守结构域进行同源性比对和进化树分析,结果表明CmbZIP18和拟南芥AtbZIP18在一个分支。组织特异性表达模式分析表明CmbZIP18在所有检测组织中均有表达,其中在成熟花粉中表达量最高,在茎中表达量最低,暗示CmbZIP18基因可能参与菊花花粉发育;结合进化树分析结果,推测CmbZIP18可能调控非生物胁迫耐性。本研究为进一步分析该基因在菊花发育及非生物胁迫应答中的功能提供了参考依据。

bZIP transcription factor proteins play an important role in plant response to abiotic stress,but the function of this family gene in Chrysanthemum has been rarely reported.In order to study the function of bZIP transcription factor in development and abiotic stress,Chrysanthemum'Jinba'was took as the experimental material,the gene sequence and expression pattern of bZIP gene CmbZIP18 were explored by qRT-PCR.The results showed that the open reading frame of the gene was 879 bp,encoding 292 amino acids,and the predicted molecular weight was 32.76 kD and the isoelectric point was 6.29.CmbZIP18 contains a conservative BRLZ domain.Subcellular localization predicted that CmbZIP18 localized in the nucleus.Conserved domains were used for homology comparison and phylogenetic tree analysis.The results showed that CmbZIP18 and AtbZIP18 were in the same branch.Tissue specific expression pattern analysis showed that CmbZIP18 was expressed in all tested tissues,with the highest expression in mature pollen and the lowest expression in stem,suggesting that CmbZIP18 may be involved in Chrysanthemum pollen development.Combined with the results of phylogenetic tree analysis,CmbZIP18 may regulate abiotic stress tolerance.This study provides a basis for furt her a nalysis of the function of this gene in Chrysanthemum development and abiotic stress response.