AgNO3对菊花叶片不定芽诱导过程中抗氧化酶活性的影响

Effect of AgNO3 on the Activity of Antioxidant Enzymes During the Induction of Adventitious Buds in Chrysanthemum Leaves

采用菊花(Dendranthema morifolium)试管苗叶片为试验材料,添加0.5 mg/L、1.0 mg/L、2.0 mg/L、4.0 mg/L AgNO3做处理,以未添加AgNO3为对照,研究不同质量浓度AgNO3对菊花叶片不定芽诱导过程中抗氧化酶活性及丙二醛含量的影响。结果表明:在处理后5~10 d期间,适宜的AgNO3质量浓度使菊花叶片超氧化物歧化酶(superoxide dismutase,SOD)活性呈急剧上升趋势,而抗坏血酸过氧化物酶(ascorbateperoxidase,APX)活性呈下降趋势,但降幅较低;在处理后10~15 d期间,0.5 mg/L AgNO3处理菊花叶片时过氧化氢酶(catalase,CAT)和过氧化物酶(peroxidase,POD)活性急剧上升,同时适宜AgNO3质量浓度能够使CAT和POD活性提前增加,使得菊花叶片防御系统正常发挥作用,在抵御Ag+造成伤害的同时,产生的过量抗氧化酶能够提前诱导不定芽的产生。较高的AgNO3质量浓度使菊花叶片膜质氧化加剧,膜系统保护酶CAT、SOD和POD活性迅速下降致使菊花叶片不定芽诱导发生时间延后以及诱导率下降。综上分析表明:0.5 mg/L AgNO3使得菊花叶片抗氧化酶活性增加,并能够提高叶片诱导不定芽的诱导率、平均芽数和一级芽率。

The leaves of chrysanthemum test-tube seedlings were treated with 0.5 mg/L,1.0 mg/L,2.0 mg/L,4.0 mg/L AgNO3,comparing with unadded AgNO3.The effects of AgNO3 on the activities of antioxidant enzymes and the content of malondialdehyde in adventitious buds of chrysanthemum leaves were studied.The results showed that the suitable mass concentration of AgNO3 caused the SOD activity of chrysanthemum leaves to rise sharply and APX activity decreased the least during the period 5~10 d after treatment.During the 10~15 d after processing,0.5 mg/L AgNO3 processing chrysanthemum leaf CAT and POD activity increased sharply,at the same time suitable AgNO3 concentration can make the CAT and POD activity increased in advance,make chrysanthemum leaf defense system normal work,can withstand the Ag+damage at the same time,the excessive antioxidant enzymes to the production of adventitious bud induction,ahead of chrysanthemum bud germination point visible CAT and POD activity in the play an important role in the process.Higher quality concentration of AgNO3 intensifies membrane oxidation of chrysanthemum leaf,and the activity of CAT,SOD and POD of membrane protective enzyme of membrane system rapidly declines,resulting in delayed time and decreased induction rate of adventitious buds in chrysanthemum leaf.In summary,the analysis showed that 0.5 mg/L AgNO3 increased the activity of antioxidant enzymes in chrysanthemum leaves and increased the induction rate,average number of buds and first-order rate of adventitious buds induced by leaves.