木霉对不同香菇菌丝体生长阶段的致病性及生防制剂对其的抑制效果

Pathogenicity of Trichoderma spp. to Lentinula edodes and The Inhibitory Effects of Biocontrol Agents

本研究通过比较两种木霉病原菌对不同香菇菌丝体生长阶段的致病性生防制剂的反应来研究木霉致病性及其防治方法。通过绿色木霉与不同香菇菌株的对峙培养结果显示,绿色木霉对不同香菇菌株的抑制效果不同,表现为对菌株‘9602’的抑制率最大;哈茨木霉则表现为不同的抑制效果,其中,对菌株‘L26’的抑制率最大。生防制剂对两种木霉的防治试验结果显示,培养48 h时的枯草芽孢杆菌杀菌剂在浓度为0.01 g/mL时对两种木霉的菌丝生长具较好的抑制效果,对绿色木霉的抑制率为76%;对哈茨木霉的抑制率为72%。多粘类芽孢杆菌对两种木霉的抑制作用也表现出差异,多粘类芽孢杆菌菌剂对哈茨木霉的菌丝生长抑制作用较绿色木霉明显,当浓度达到0.001 g/mL时对两种木霉菌丝生长均具有较好的抑制作用,对哈茨木霉的抑制率为75%,高于绿色木霉(抑制率为38%)。综上所述,尽管不同木霉菌株的致病性有差异,但总体表现为木霉对香菇菌丝具较强的致病性。两种生防药剂对不同木霉菌株具有不同的防治效果,为生防制剂在食用菌病害生物防治提供理论依据。

The pathogenicity of Trichoderma spp.and its control methods were studied by comparing the pathogenicity of two Trichoderma pathogens and the response to two biocontrol agents.The inhibiting effects of Trichoderma viride and T.harziamum on the different Lentinus edodes strains showed different,the effect of T.viride on the strain'9602'was the best.The inhibition effect of T.harzianum on the strain'L26'is the highest.The prevention and control results of biocontrol preparations on two kinds of Trichoderma showed that the control effects of Bacillus subtilis fungicide when the concentration is 0.01 g/mL on T.viride and T.harzianum were all good.The effect on the T.viride is 76%and the effect on the T.harziamun is 72%after cultured 48 h.The control effects of Paenibacillus polymyxa on T.viride and T.harzianum were difference,the effects when the concentration is 0.001 g/mL on T.viride is 75%,higher than the T.harzianum(38%).In summary,Trichoderma strains had strong pathogenicity on mushroom strains even though different Trichoderma strains have different pathogenicity on different strains of Lentinus edodes,two biocontrol agents have excellent control effects on different Trichoderma strains,which would provide thoery basis for the edible mushroom disease biological control.